John H. Cross
Disease manifestations include inflammation of lymph nodes (lymphadenitis), irregular fevers, and lymphedema. Repeated, chronic infection may result in elephantiasis.
Adults are elongate and threadlike. Microfilariae are 250 to 300 µm long, equal in diameter to a red blood cell, and sheathed.
Filariae are nematodes (roundworms). They are vectored by arthropods; mature and mate in specific host tissues; and produce microfilariae. The lymphatic filariae Wuchereria bancrofti and Brugia malayi reside in lymphatics.
Multiplication and Life Cycle
Adult female worms produce microfilariae. Feeding vector mosquitoes ingest microfilariae from the bloodstream. In the mosquito the microfilariae mature to infective larvae, which migrate to the mosquito's mouth-parts, enter a new host via the vector's puncture wound, migrate to the lymphatics, mature, and mate.
Disease manifestations are due to lymphatic dysfunction resulting from the presence of living and dead worms, lymph thrombi, inflammation, and immune reactions to worms and worm products.
Inflammatory reactions result in cellular infiltration and fibrosis of lymphatics. Damage to vessel walls result in endothelial cell proliferation. It is not yet clear to what extent circulating antibodies to filariae are protective.
Lymphatic filariasis is prevalent in many tropical and subtropical countries where the vector mosquitoes are common.
Diagnosis is suggested clinically by lymphangitis and irregular fever; definitive diagnosis depends on demonstrating microfilariae in thick blood smears.
There is no consistently effective treatment to kill adult worms. Control is by avoiding or reducing vector mosquitoes and by treating individuals with microfilariacides.
Patients present with subcutaneous nodules, dermatitis, and eye lesions.
Long, thread-like adult worms live coiled in subcutaneous nodules and produce microfilariae that are slightly smaller than those of lymphatic filariae and are not sheathed.
Onchocerca volvulus is a typical filarial nematode that is primarily a parasite of humans.
Multiplication and Life Cycle
Adult worms live in subcutaneous nodules. Microfilariae wander through the skin, where they may be picked up by a feeding vector blackfly. In the blackfly they mature to infective larvae, which may enter a new host when the blackfly feeds. The larvae then move to the subcutaneous tissues, mature, mate, and produce microfilariae.
Reaction to worms and microfilariae causes dermatitis with loss of skin elasticity and the formation of fibrotic subcutaneous nodules. Living and dead microfilariae in the eye cause trauma and reactions that can result in blindness.
There is an inflammatory and immune response to living and dead parasites and to antigen-antibody complexes. Adult worms are localized in the subcutaneous tissues, surrounded by fibrotic nodules and ultimately calcify.
Onchocerciasis is common in Africa; foci also occur in South and Central America and Mexico. The blackfly vectors breed in oxygen-rich water; thus, the disease characteristically is associated with fast-flowing streams.
The clinical picture is indicative; final diagnosis is made by identifying microfilariae in skin snips.
Individuals may be treated by removal of nodules and by administering microfilariacides that kill microfilariae. Control of vector blackflies minimizes reinfection.
Several other minor filarial nematodes parasitize humans; the pathology depends on the tissues preferred by the worms. In addition, humans occasionally are infected with dog heartworm larvae, which are unable to mature in human tissues but can cause lesions.
The filariae are thread-like parasitic nematodes (roundworms) that are transmitted by arthropod vectors. The adult worms inhabit specific tissues where they mate and produce microfilariae, the characteristic tiny, thread-like larvae. The microfilariae infect vector arthropods, in which they mature to infective larvae. Filarial diseases are a major health problem in many tropical and subtropical areas. The disease produced by a filarial worm depends on the tissue locations preferred by adults and microfilariae. The adults of the lymphatic filariae inhabit lymph vessels, where blockage and host reaction can result in lymphatic inflammation and dysfunction, and eventually in lymphedema and fibrosis. Repeated, prolonged infection with these worms can lead to elephantiasis, a buildup of excess tissue in the affected area. Other filariae mature in the skin and subcutaneous tissues, where they induce nodule formation and dermatitis; migrating filariae of these species can cause ocular damage. Table 92-1 summarizes the filarial infections of humans.
Lymphatic Filariae Wuchereria Bancrofti and Brugia Malayi
In lymphatic filariasis, disease is caused by the presence of worms in the regional lymphatic vessels and, particularly, by the host response to the worms and worm products. The microfilariae are released into the blood. Infections involving small numbers of worms are often asymptomatic. Early symptoms of lymphatic filariasis consist of intermittent fever and enlarged, tender lymph nodes. The inguinal lymph nodes are very often involved. The lymphatic vessels that drain into the lymph nodes and that harbor the developing and adult worms also become inflamed and painful. In more chronic infections, there may be pain also in the epididymis and testes. Swollen lymphatics may burst and drain into the genitourinary system; the resulting chyluria is sometimes the symptom that brings the patient to a doctor.
In a small number of chronic cases, permanent lymphatic dysfunction caused by repeated exposure to infection over a number of years results in the massive lymphedema and accumulation of excess tissue known as elephantiasis.
Structure and Classification
Adult Wuchereria and Brugia are elongated and slender (30 to 100 mm by 100 to 300 µm); males are about half the size of females. Microfilariae are the diameter of a red blood cell and 250 to 300 µm long. They are enclosed in a characteristic sheath. In addition to B malayi, some other, related Brugia species can infect humans or animals; they resemble B malayi in structure and life cycle, and are not discussed here. B timori, found only in Indonesia, is similar to B malayi, but is larger.
Multiplication and Life Cycle
The filariae require an intermediate arthropod host to complete their life cycle (Fig. 92-1). However, no multiplication takes place in the intermediate host. Adult male and female worms live in regional lymphatic vessels, where the female produces a large number of microfilariae, which circulate in the blood and may be ingested by a feeding vector mosquito. The female worms show a circadian periodicity in microfilaria production. The time of peak production varies among the species and geographic strains of worms and usually corresponds to the peak feeding period of the vector mosquitoes. Microfilariae ingested by a vector mosquito migrate out of the midgut to the thoracic muscles, where they develop, molt several times, and finally migrate to the mouthparts of the mosquito as infective larvae. As the infective mosquito feeds on another host, the larvae leave the proboscis and enter the puncture wound made by the mosquito. Larvae quickly migrate to the lymphatics, where they mature, mate, and produce microfilariae in the new host. The time from infection until microfilariae can be detected in the blood varies from 3 to many months. There is no reliable information on the average life span of adult worms; however, humans who leave endemic areas have been observed to have circulating microfilariae for several years.
FIGURE 92-1 Life cycle of Wuchereria and Brugia
Infective larvae from a feeding vector mosquito migrate to the regional lymphatic vessels and by inducing a host inflammatory reaction cause the eventual blockage and edema characteristic of W bancrofti and B malayi infections (Fig. 92-1). The pathology varies greatly from one individual to another, and the exact mechanisms are not completely understood. The host reaction to the parasite is considerable and worsens when the worms molt, when the females first begin to produce microfilariae, and when the worms die and degenerate. Lymphatic vessels are often partially or completely blocked by lymph thrombi, by masses of dead worms, or by endothelial proliferation, fibrin deposition, and granulomatous reactions. Lymph stasis favors secondary bacterial and mycotic infection. The initial inflammation of regional lymph nodes and major lymphatic vessels may be followed by a prolonged asymptomatic period and then by recurring attacks of lymphangitis and "filarial fever" over a period of years. Figure 92-2 shows an example of elephantiasis, a grotesque enlargement of the infected area that develops when recurring attacks of lymphangitis result in permanent lymphatic blockage and lymphedema. The exact cause of elephantiasis is not understood; however, repeated exposure appears to lead to production of abnormally large amounts of collagenous material and to fibrosis of the tissue around the affected lymphatics.
FIGURE 92-2 Elephantiasis of leg caused by chronic infection with the filarial nematode Wuchereria bancrofti. (Courtesy of Shoyei Yamauchi, Honolulu, HI.)
The reaction to developing and adult worms results in endothelial cell proliferation and thrombus formation within the lymphatic vessels. Some aspects of the wide disease spectrum seen in lymphatic filariasis can be correlated with host immune responses. Individuals living in endemic areas and frequently bitten by infective mosquito vectors vary in clinical manifestations. Patients with characteristic symptoms such as recurring fever and regional lymphangitis may or may not have microfilariae in peripheral blood. Although antibodies to microfilariae and adult worms may be present, they do not seem to prevent reinfection or disease, since in endemic areas the proportion of exposed individuals who have microfilariae or clinical symptoms may increase until middle age. As a general rule, adults not previously infected with lymphatic filariae will show symptoms, but not circulating microfilariae, following infection with large numbers of infective larvae. Patients with elephantiasis also usually do not have circulating microfilariae. They do, however, have high levels of antibody to microfilariae and increased lymphocyte proliferative responses to adult worm antigens.
Wuchereria bancrofti is prevalent in many parts of the tropics and subtropics. Species from three major genera of mosquitoes that serve as vectors are the common house mosquito Culex pipiens quinquefasciatus (C fatigans) in many urban centers, Aedes species in the South Pacific islands, and Anopheles species in more isolated rural areas. The relation between the degree and frequency of infection and the development of the disease is still not fully understood; severe disease such as elephantiasis develops gradually as a result of repeated exposure. Circulating microfilariae may persist for many years in the absence of specific symptoms.
Humans previously were assumed to be the only hosts for W bancrofti; however, studies have shown that several species of monkeys can be infected experimentally with infective stage W bancrofti larvae recovered from mosquitoes allowed to feed on infected human volunteers. No naturally infected reservoirs other than humans have been identified.
Brugia malayi differs somewhat from W bancrofti in epidemiology. Whereas W bancrofti is prevalent in tropical areas all over the world, B malayi is found mainly in Southeast Asia. In contrast to W bancrofti, which is transmitted by mosquitoes of the three major genera, the principal mosquito vectors of B malayi belong to the genus Mansonia. B malayi is less host-specific than W bancrofti; it has been recovered from naturally infected monkeys, cats, and dogs, and has been maintained in several laboratory animals.
Enlarged and tender lymph nodes, especially in the inguinal region, or inflammation of lymphatic vessels in the extremities should alert physicians in an endemic area to filariasis. Definitive diagnosis may be accomplished by identifying microfilariae in thick blood smears (Fig. 92-1). Species identification is based on the presence of a sheath and the position of terminal nuclei and the size of the cephalic space. Because of the nocturnal periodicity of microfilariae, blood smears are better made at night when microfilarial levels are usually higher. Light infections may be detected by using one of several concentration methods.
Unfortunately, microfilariae may not be present in the blood during the early and late stages of the disease. When microfilariae are not detectable, a history of recurrent episodes of lymphangitis and lymphadenitis may form the basis for a presumptive diagnosis. Skin tests have been largely unsatisfactory, and commercial antigen is not widely available. Serologic tests are useful in epidemiologic studies, but to date have had limited value in the management of individual cases. Molecular cloning of antigens and antigen capture techniques are showing promise. New developments in ultrasonography and lymphoangioscintigraphy may also contribute to diagnosis.
Attempts to reduce the prevalence of lymphatic filariae include vector control and mass treatment campaigns using diethylcarbamazine citrate. Some mass treatment programs have used table salt medicated with diethylcarbamazine. This drug significantly reduces the level of microfilariae in the blood. However, it must be given over a prolonged period, and frequent side effects, such as fever, vertigo, headaches, nausea, and lymphatic inflammation, discourage patient cooperation. Ivermectin, a drug that has recently been shown to be effective in the treatment of onchocerciasis, is being evaluated for use in lymphatic filariasis. Neither drug is very effective at killing adult worms. Combinations of diethylcarbamazine and ivermectin are being tested. In some areas single doses of these drugs in combination or singly are also being evaluated. Fewer side effects are experienced with the shorter treatments.
Onchocerca volvulus is a filarial worm that is transmitted to humans by blackflies (Simulium). Mature worms live in the subcutaneous tissues and produce microfilariae that migrate through the skin and connective tissues.
Changes in skin pigmentation are often the first obvious signs of Onchocerca infection. Later stages of dermatitis present as atrophy and loss of skin elasticity. Although lymph nodes may become involved in onchocerciasis, involvement is not as prominent as with the lymphatic filariae. The subcutaneous nodules that harbor adult Onchocerca are usually firm and non-tender. They vary in size and location but usually are easily recognized when they occur in geographic areas where the disease is endemic. However, in some geographic regions, nodules may be in deeper tissue, and thus not easily palpable.
The most serious clinical manifestation of onchocerciasis is blindness, caused by microfilariae that wander into the eye. In endemic areas, corneal opacities resulting from the reaction to dying microfilariae often suggest onchocercal infection. Alternatively, active living microfilariae may be seen when the eye is examined with a slit lamp.
Adult Onchocerca may be up to 60 cm long, but are usually coiled in subcutaneous nodules. The microfilariae are slightly smaller than those of W bancrofti and B malayi and differ from them in lacking a sheath, having a different nuclear arrangement, and not usually being found in the blood.
Multiplication and Life Cycle
Figure 92-3 shows the life cycle of Onchocerca. The microfilariae produced by adult female worms in subcutaneous nodules migrate into the skin and connective tissue; they do not generally enter the circulatory system. Microfilariae are ingested by vector blackflies, develop to the infective stage in the muscles of the flies, and then migrate to the mouth parts. When the infected flies feed on a new host, the larvae leave the mouth parts and enter the wound produced by the biting fly. Developing male and female worms congregate in subcutaneous tissue where they usually induce formation of a nodule.
FIGURE 92-3 Life cycle of Onchocerca.
Adult worms in the subcutaneous tissues cause varying degrees of inflammation and may induce subcutaneous nodules. Nodules appear 3 to 4 months after infection, but microfilariae are not generally detectable until I year after infection. Adult worms may be surrounded by an inflammatory response that progresses to granuloma formation and fibrosis or calcification, depending on the condition of the worm and age of the nodule (Fig. 92-3).
Microfilariae appear to move upward, and in chronic heavy infections may be seen in the eye. Ocular damage is thought to be due both to the trauma caused by living microfilariae and to a hypersensitivity reaction to dead ones. A major problem in the management of onchocerciasis patients is the acute inflammatory response to dying microfilariae in the eye during treatment. Antigen-antibody complexes probably play a role in the development of eye lesions resulting from microfilariae.
Nodules containing adult worms are surrounded by inflammatory cells that are replaced by collagen and fibrotic tissue, thereby localizing the worms. A study of nodules has shown that they are areas of high cellular activity. Some microfilariae appear to be killed before they ever leave the nodules where they are produced by the female worm. Obviously many others escape the chronic inflammatory responses and the granulomas commonly seen surrounding nematodes in tissue. The exact composition of the nodules varies, depending on the distance from the adult worms and the age of the nodule. In general, neutrophils are followed by eosinophils and macrophages. The involvement of eosinophils in killing microfilariae in untreated patients suggests specific immune reactions similar to those reported in other helminth infections. The fibrous outer layer of the nodule contains blood vessels, which may be surrounded in older nodules by cellular infiltrates that include plasma cells and eosinophils.
Transmission occurs through bites of vector blackflies in the family Simuliidae. Although a few vector species breed in slow-moving streams, most require fast-flowing, highly oxygenated streams or rivers. For this reason, ocular onchocerciasis is often called "river blindness."
Onchocerciasis is prevalent in many parts of tropical Africa and has been reported in a few places in the Middle East. In the Western hemisphere, it is an important and widespread infection in Guatemala and the southern states of Mexico. It also appears in other areas of Central America, and a few foci have been found in Venezuela, Colombia, Surinam, Brazil, and Ecuador.
Onchocerciasis is suggested by subcutaneous nodules or by the characteristic scaly dermatitis in individuals living in endemic areas. Positive diagnosis is usually made by identifying microfilariae in a superficial skin biopsy made with a scalpel or an appropriate punch. The skin samples, which are usually taken from the shoulder, are placed in a drop of saline or distilled water on a microscope slide, incubated for 30 minutes, and examined (Fig. 92-3). The diagnosis may also be made by finding adult worms in nodules or by observing microfilariae in the eye. Serologic tests are being developed to aid in diagnosis when microfilariae cannot be detected but onchocerciasis is suspected.
Three main measures - vector control, nodule removal, and drug treatment - provide limited control of onchocerciasis. Control of blackfly vectors is difficult because most species breed in fast-flowing streams to which insecticides cannot easily be applied. Nodules harboring adult worms are usually removed, which presumably reduces the rate at which microfilariae are produced and thus the risk to the eyes (as well as the number of microfilariae available to vectors). Mass treatment poses difficulties, because suramin, the drug of choice for killing adult worms, must be given intravenously and is toxic. Diethylcarbamazine kills microfilariae but has limited efficacy against adult worms and has the disadvantage that some patients experience a severe reaction, which may exacerbate eye damage, to dying microfilariae. Ivermectin, a semisynthetic macrolide antibiotic, has recently been shown to be more effective and to produce fewer side effects than diethylcarbamazine.
Minor Filarial Infections
Human filarial parasites of minor importance include the following (Table 92-1):
Loa loa, limited in distribution to tropical Africa, best known for its superficial migration under the conjunctiva of the eye and for the presence of "fugitive" or "calabar" temporary swellings
Mansonella (syn. Dipetalonema or Acanthoceilonema) streptocerca, also limited to tropical Africa and frequently presenting as a chronic itching dermatitis; both adult worms and microfilariae are present in the skin
Mansonella (syn. Dipetalonema or Acanthoceilonema) perstans, found in Africa and South America
Mansonella ozzardi, restricted to the western hemisphere
Dirofilaria immitis (the dog heartworm) is a worldwide filarial parasite of dogs; adult worms (up to 30 cm long) usually are located in the dog's heart. In heavy infections, or when adult worms die, the parasites may be carried to the pulmonary vessels where they may produce emboli. The worms do not mature to adulthood in humans, but larval stages have been reported in cutaneous nodules (which may be confused with tumors) and have sometimes produced lesions in the lungs (coin lesions) or in breast tissue. Pulmonary lesions may be asymptomatic or may cause coughing or chest pains. Roentgenograms frequently show a discrete mass suggestive of a tumor. Larvae of other Dirofilaria spp. that parasitize lower animals occasionally may be found in the skin or eyes of humans.
Dracunculus medinensis, the guinea worm, is not a true filarial worm, but is often grouped with the filariae. It is one of the oldest known parasitic diseases of man. Some authors suggest that the ancient technique of removing the adult female worm, which may be more than 1 meter long, by winding it on a stick, may be the origin of the medical emblem, the caduceus.
Dracunculiasis is still quite common in parts of Africa and Asia. Humans acquire the infection when they swallow infected copepods (genus Cyclops) in drinking water. Often, the first sign of infection is the formation of a blister when the adult female worm migrates to the subcutaneous tissue. On contact with water, the blister breaks and the worm ruptures and discharges larvae into the water. The larvae are ingested by Cyclops, in which they develop to the infective stage.
The ultimate location of the adult worm determines the pathology, since lesions develop in response to the worms. Frequent sites of inflammation and abscess include knee and ankle joints, as well as the subcutaneous tissues of the extremities.
Amaral F, Dreyer G, Figueredo-Silva J, Noroes J, Cavalcanti A, Samico SC, Santos A, Coutinho A: Live adult worms detected by ultrasonography in human bancroftian filariasis. Am J Trop Med Hyg 50:753, 1994
Cross JH, Partona F, Hsu MYK, Ash LR, Oemijati S: Experimental transmission of Wuchereria bancrofti to monkeys. Am J Trop Med Hyg 28:55, 1979
Cross JH: Recent advances in epidemiological field techniques in filariasis. Southeast Asian J Trop Med Pub Hlth 24(Suppl)40, 1993
Freedman DO, deAlmeido Filho PJ, Besh S, Maiaes Silva MC, Braga C, Maciel AJ: Lymphoscintigraphic analysis in lymphatic abnormalities in symptomatic and asymptomatic human filariasis. J Infect Dis 170:927,1994
Gelband H: Diethylcarbamazine salt in the control of lymphatic filariasis. Am J Trop Med Hyg 50:655, 1994
Moulia-Pilot JP, Glaziou P, Nguyen LN, Chanteau S, Martin PM, Cartel JL. Long-term efficacy of a single dose treatment with 400 micrograms.kg-1 of ivermectin in bancroftian filariasis: results at one year. Trop Med Parasitol 44:333, 1993
Tawill SA, Kipp W, Lucius R, Gallin M, Erttmann KD, Buttner DW: Immunodiagnostic studies on Onchocerca volvulus and Mansonella perstans infections using a recombinant 33 kDa O volvulus protein (Ov33). Trans Roy Soc Trop Med Hyg 89:51, 1995
Turner PF, Rockett KA, Ottesen EA, Francis H, Awadzi K, Clark IA: Interlukin-6 and tumor necrosis factor in the pathogenesis of adverse reactions after treatment of lymphatic filariasis and onchocerciasis. J Infect Dis 169:1071, 1994
Wamae CN, Advances in the diagnosis of human lymphatic filariasis: a review. East Afr Med J 71:171,1994
World Health Organization: Lymphatic Filariasis: The disease and its Control. WHO Tech Rpt Ser 821, Geneva, 1992