METHODICAL INSTRUCTION FOR STUDENTS OF THE 3-d COURCRSE

Pharmaceutical faculty

Practical class №25 (6 hours)

Theme: Analysis of species (herbal composition, collection)

Aime: study to determine the authenticity of spicies.

Professional orientation. Medicinal species (collections) and teas are mixtures of several kinds of cut herbal raw materials, except of drastic herbs. Teas are used primarily for internal use, and collections - for gargles, lotions and poultices. For the preparation of collections and tea medical raw material should be grounded. Rhizomes and roots cut and passed through grinders. Pods and seeds as well as skinny leaves of bearberry, cranberry and eucalyptus are pounded until coarse material or can be passed through the rollers or the mill. Berries and flowers except lime flowers and camomile flowers are used whole. Leaves, herb and bark are cut.

Chopped (crushed) and sieved through the metal sieve herbal crude drug is mixed to the uniform mixture.

Species and teas are investigated with magnifying glass and microscope.

In folk medicine species are used very often. One plant can increase and enforce the action of the others.

Basic knowledge and skills:

1. Plant systematization (“Medical Botany” 2^nd course)

2. Anathomy and morphology of plants (“Medical botany”, 1^st course)

3. Physical and chemical properties of saponins (“Pharmacognosy”, 3rd course).

Methodology of Practical Class (9⁰⁰- 12⁰⁰)

Materials and equipment: samples of species, medicinal plant materials, microscopes, magnifiers, preparative needles, forceps, subject and cover glass, analysing board.

Work 1 Study species.

Work 2 Conduct the analysis of species.

Each student receives the sample of the unknown species and analysis should be provided by the scheme:

1. Determination of the components of the species. Place the species on the clean flat surface and divide all components by general appearance using magnifier. Check with your teacher the number of components.

2. Determination of authenticity of whole MPM by tables.

3. Take 5-10 similar pieces of the MPM and make micropreparations according to the Eu Ph 6.0. Study their anatomical structure and determine the names.

4. Show the diagnostic features to your teacher, and check the accuracy of the determination.

5. Write your results to the protocol:

a) describe the general appearance, colour and odour of the species and of its components;

b) draw and sign diagnostical features of the components;

c) Note the results of chemical and micro-chemical reactions;

d) Write the content of the species and its pharmacological effects.

Seminar discussion of theoretical issues (12³⁰- 14⁰⁰)

1. Specify the main components of species?

2. What are medicinal plant materials used the most often?

3. According to which scheme are MPM analysed?

Test evaluation and situational tasks

1. The collection consists of wormwood herb. What is the species? At what symptoms can this medicine be recommend?

Student should know:

1. Medicinal plant materials that used in herbal composition

2. The main components of species

Student should be able

1. The methods of macro- and microscopic analysis of the MPM.

2. Analysis of the collections.

Correct answers of test evaluations:

Wormwood herb is in the appetizing medicinal collection, which recommend for appetite stimulation.

References

A- Main:

1. Solodovnichenko N.M., Zhuravlev M.S., Kovalev V.M. Medicinal plant raw materials and herbal medicines / Manual for Pharmacognosy with the fundamentals of biochemistry of medicinal plants. - Kharkov: Golden Pages.-2001.-P.120-131.

2. Pharmacognosy / [V. S. Kyslychenko, L. V. Upyr, Ya. V. Dyakonova etc.]; edited by V. S. Kyslychenko. ― Kharkiv : NUPh «Golden Pages», 2011. ― 551 p.

3. Pharmacognosy laboratory hand-book / V. S. Kislichenko, L. V. Upyr, I. O. Zhuravel, V. V. Velma etc. ― Kharkiv : NUPh, 2011. ― 551 p.

4. Antonyuk V. O. A Laboratory manual on Pharmacognosy /V. O. Anthonyuk, Lysyuk R. M., Anthonyuk L. Ya. ― Lviv, 2012. ― 220 p.

5. Antonyuk V. O. A Practical course of Pharmacognosy /V. O. Anthonyuk, Lysyuk R. M., Anthonyuk L. Ya. ― Lviv, 2011. ― 499 p.

6. European Pharmacopoeia / Council of Europe ― 6.0 edition. ― Strasbourg, 2008. ― Vol. 1, 1084 p.

7. European Pharmacopoeia / Council of Europe ― 6.0 edition. ― Strasbourg, 2008. ― Vol. 2.1., 2200 p.

8. European Pharmacopoeia / Council of Europe ― 6.0 edition ― Strasbourg, 2008. ― Vol. 2.2., 3308 p.

9. William Charles Evans Pharmacognosy / William Charles Evans. ― [16^th edition] ― China : Elsevier, 2009. ― 603 p.

10. University Web – site > Intranet > Students facilities > Practical classes meterials > Department of Pharmacognosy with Medical Botany > English > Pharmaceutical faculty > Pharmacy > Full time study > Pharmacognosy > 3-d course >Analysis of species.

B- Additional:

1. Andrew Pengelly Constituents of Medicinal Plants / Andrew Pengelly. ― [2^nd edition] ― CABI Publishing, 2009. ― 172 p.

2. World health organisation on selected medicinal plants ― Berlin, 1999. ― Vol. 2, 352 p.

3. World health organisation on selected medicinal plants ― Geneva, 2007. ― Vol. 3, 376 p.

Methodical instruction has been worked out by: assistant Kozachok S.S.

Methodical instruction was discussed and adopted at Pharmacognosy

with Medical Botany department sitting 27 August 2012, minute № 1