EMULSIONS.
An emulsion is
essentially a liquid preparation containing a mixture of oil and water that is
rendered homogeneous by the addition of an emulsifying agent. The emulsifying agent ensures that the oil phase is finely
dispersed throughout the water as minute globules (Figure 4.1). This
type of emulsion is termed an ‘oil-inwater’ emulsion. The oily phase (disperse phase) is dispersed through the aqueous phase (continuous phase). Generally
all oral dose emulsions tend to be oil-in-water as the oily phase
is usually less pleasant to take and more diffi
cult to fl avour. ‘Water-in-oil’ emulsions
can be formed but these tend to be those withexternal uses.
Introduction and
overview of emulsions
The pharmaceutical
term ‘emulsion’ is solely used to describe preparations
intended for internal use, i.e. via the oral route of administration.
Emulsion formulations for external use are always given a different
title that refl ects their
use, e.g. application, lotion and cream
Figure 4.1 Illustration of an oil-in-water
emulsion..
British
Pharmacopoeia (BP) definition (oral emulsions)
Oral
emulsions are oral liquids containing one or more active ingredients. They are stabilized oil-in-water dispersions, either or both phases of which may contain dissolved solids. Solids may also be suspended in oral emulsions. When issued for
use, oral emulsions should be supplied in wide-mouthed
bottles.
Extemporaneous preparation
In
oral emulsions prepared according to the formula and
directions given for extemporaneous preparation,
the quantity of emulsifying agent specified
in individual monographs may be reduced to
yield a preparation of suitable
consistency provided that by so doing the
stability of the preparation is not adversely
affected.
Advantages and disadvantages of emulsions as dosage forms
Advantages
-
Unpalatable oils can be administered in palatable
form.
-
Unpalatable oil-soluble drugs can be administered in palatable form.
-
The aqueous phase is easily flavoured.
-
The oily sensation is easily removed.
-
The rate of absorption is increased.
-
It is possible to include two incompatible ingredients, one
in each phase of the emulsion.
Disadvantages
-
Preparation needs to be shaken well before use.
-
A measuring device is needed for administration.
-
A degree of technical accuracy is needed to measure a dose.
-
Storage conditions may affect stability.
-
Bulky, difficult to transport and prone to container breakages.
-
Liable to microbial contamination which can lead to cracking.
Stability of emulsions
Emulsions
can break down in the following ways:
-
cracking
-
creaming
-
phase inversion.
Cracking
This
is the term applied when the disperse phase
coalesces and forms a separate layer. Redispersion cannot be achieved by shaking and the preparation is no longer an emulsion. Cracking can occur if the oil turns rancid during storage. The acid formed denatures the emulsifying agent, causing the two phases to separate.
Creaming
In creaming, the
oil separates out, forming a layer on top of the emulsion, but it
usually remains in globules so that it can be redispersed on shaking (e.g. the cream on the top of a pint of milk). This is undesirable as the product appearance is poor and if the product is not adequately shaken there is a risk of the patient obtaining an incorrect dose. Creaming is less likely to occur if the viscosity of the continuous phase is increased.
Phase inversion
This is the
process when an oil-in-water emulsion changes to a water-in-oil
emulsion or vice versa. For stability of an emulsion, the optimum range
of concentration of dispersed phase is 30–60% of the total
volume. If the disperse phase exceeds this, the stability of
the emulsion is questionable. As the concentration of the disperse phase
approaches a theoretical maximum of 74% of the total
volume, phase inversion is more likely to occur.
Summary of the problems encountered by emulsions
Creaming
Separation of the emulsion into two regions, one containing
more of the disperse phase.
Possible reasons
for problem
-
lack of stability of the system.
-
product not homogeneous.
Can the emulsion
be saved?
The emulsion will
reform on shaking.
Cracking
The globules of
the disperse phase coalesce and there is separation of the disperse phase
into a separate layer.
Possible reasons
for problem:
-
incompatible emulsifying agent
-
decomposition of the emulsifying agent
-
change of storage temperature.
Can the emulsion
be saved?
The emulsion will
not reform on shaking.
Phase inversion
From oil-in-water
to water-in-oil or from water-in-oil to oil-in-water.
Possible reason
for problem
-
amount of disperse phase greater than 74%.
Can the emulsion
be saved?
The emulsion will
not reform on shaking
General method
The theory of emulsifi cation is based on the study of the most naturally occurring emulsion, milk. If
examined closely, milk will be seen to consist of
fatty globules, surrounded by a layer of casein, suspended in water. When a
pharmaceutical emulsion is made, the principal
considerations are the same. The object is to divide
the oily phase completely into minute globules, surround each globule with
an envelope of suspending agent (e.g. Acacia BP) and fi nally suspend the globules in the aqueous phase (Figure 4.1). As with other
liquid preparations for oral use, emulsions will have in the formulation a
vehicle containing added flavouring or colourings
as required. There is also the need for a preservative, which is usually chloroform, in the form of Double Strength
Chloroform Water BP. In addition an emulsion will also need an
emulsifying agent (or emulgent).
Continental and dry gum method
Although emulsions
may be made by a variety of methods (for example, using
methylcellulose gum in the preparation of Liquid Paraffi n Emulsion BP:
see Example 4.4 below), extemporaneously prepared emulsions
for oral administration are usually made by the continental or
dry gum method, where the emulsion is formed by mixing the
emulsifying gum (usually Acacia BP) with the oil which is then
mixed with the aqueous phase. The only differences between the
continental and dry gum methods are the proportions of
constituents within the primary emulsion (for example, fixed-oil emulsions made by the continental method would use a
ratio of 4:3:2 rather than 4:2:1 with
the dry gum method). Internal emulsions prepared by
the dry gum method should contain, in addition
to the oil to be emulsified:
-
a vehicle
Freshly
boiled and cooled purifi ed water is normally used because of the increased risk from microbial
contamination.
-
a preservative
This
is usually added to the product as Double Strength Chloroform Water BP at 50% of the volume of the
vehicle. If freshly boiled and cooled purifi ed
water is used as the vehicle, it would be
appropriate to manufacture the Double Strength Chloroform Water BP using freshly boiled and cooled
purified water rather than potable
water.
-
an emulsifying agent (or emulgent)
The
quantity of emulsifying agent added is determined by the type of oil to be emulsifi ed and the quantity of emulsion to
be prepared
-
additional flavouring if
required
-
additional colouring if required
Calculation of the amount of
emulsifying agent to be usedin the preparation of an
emulsion
The
amount of emulsifying agent used is dependent on the amount and type of oil to be emulsifi
ed. Oils can be divided into three categories:
fi xed oils, mineral oils
and volatile oils.
Fixed
oils
Oil: 4 parts by volume
Aqueous phase: 2 parts by volume
Gum: 1 part by weight
Mineral
oils
Oil: 3 parts by volume
Aqueous phase: 2 parts by volume
Gum: 1 part by weight
Volatile
(aromatic) oils
Oil: 2 parts by volume
Aqueous phase: 2 parts by volume
Gum: 1 part by weight
These
proportions are important when making the primary emulsion, to prevent the emulsion breaking down on
dilution or storage.
The quantities for
primary emulsions (in parts) are summarised
in the key points box.
Wet gum method
The proportions of
oil, water and emulsifying agent for the preparation of
the primary emulsion are the same as those used in the dry gum method.
The difference is in the method of preparation. Using this method the acacia powder is added to the
mortar and then triturated with the water until the gum is
dissolved and a mucilage
formed. The oil is then added to the mucilage drop by drop
whilst triturating continuously. When nearly all the oil has been added the
resulting mixture in the mortar may appear a little poor
with some of the oil appearing to be absorbed. This can be rectifi ed
by the addition of slightly more water. The trituration
continues until all the oil has been added, adding extra small amounts of water
when necessary. When all the oil has been added triturate
until a smooth primary emulsion is obtained. In the main, this method has
fallen out of favour as it takes much longer than
the dry gum method. It should be noted that there is less chance of
failure with this method provided the oil is added very
slowly and in small quantities. It also means that the reasons for
failure when using the dry gum method (outlined above)
have been eliminated.
Clean, dry equipment
All equipment
should be thoroughly cleaned, rinsed with water and carefully dried before
use, particularly measures, mortars and pestles.
Accurate quantities
Accurate
quantities are essential. Check weighing/measuring technique and minimize transference losses; for
example, allow oil to drain from measure.
Have all ingredients ready
Correct rate of
addition is important. Ingredients for the primary emulsion should all be weighed and measured before starting to make the product.
General method of preparation of an emulsion using the dry gum method
It is relatively
easy for an emulsion to crack, resulting in a failed product.
Remember that the key points opposite are critical when preparing
emulsions.
The preparation of
an emulsion has two main
components:
1. preparation of a concentrate called the primary emulsion
2. dilution of the concentrate.
Preparation of the primary emulsion
1. Measure the oil accurately in
a dry measure. Transfer the oil into a large dry porcelain
mortar, allowing all the oil to drain out.
2. Measure the quantity of
aqueous vehicle required for the primary emulsion. Place this
within easy reach.
3. Weigh the emulsifying agent
and place on the oil in the mortar. Mix lightly with the
pestle, just suffi cient to
disperse any lumps. Caution: overmixing generates heat,
which may denature the emulsifying agent and result in a poor product.
4. Add all of
the required aqueous vehicle in one addition. Then mix vigorously, using the pestle with a shearing action in one direction.
5. When the product becomes white
and produces a clicking sound, the primary emulsion
has been formed. The product should be a thick, white
cream. Increased degree of whiteness indicates a better-quality
product. Oil globules or slicks should not be apparent.
Dilution of the primary emulsion
1. Dilute the primary emulsion
drop by drop with very small volumes of the remaining
aqueous vehicle. Mix carefully with the pestle in one direction.
2. Transfer emulsion to a
measure, with rinsings. Add other liquid ingredients if necessary and make up to the final volume.
Emulsions are biphasic systems consisting of two immiscible liquids, one of which (the dispersed phase) is finely subdivided and
uniformly dispersed as droplets throughout the other phase (the dispersion medium).
1. They can mask the bitter taste and odor of drugs, thereby making them more palatable. e.g.
castor oil, cod-liver oil etc.
2. They can be used to prolong the release of the drug
thereby providing sustained release action.
3. Essential nutrients like carbohydrates, fats and vitamins can all be emulsified and can be administered to bed
ridden patients as sterile intravenous emulsions.
4. Emulsions provide protection to drugs which are
susceptible to oxidation or hydrolysis.
5. Intravenous emulsions of contrast media have
been developed to assist in diagnosis.
6. Emulsions are used widely to formulate
externally used products like lotions, creams, liniments etc.
DIFFERENCE BETWEEN O/W AND W/O EMULSIONS
S.No |
Oil in water emulsion (o/w) |
Water in oil emulsion (w/o) |
1. |
Water is the dispersion medium and oil
is the dispersed phase |
Oil is the dispersion medium and water
is the dispersed phase |
2. |
They are non greasy and
easily removable from the skin
surface |
They are greasy and not
water washable |
3. |
They are used externally to provide cooling
effect e.g. vanishing cream |
They are used externally to prevent evaporation
of moisture from the surface
of skin e.g. Cold cream |
4. |
Water soluble drugs are more
quickly released from o/w emulsions |
Oil soluble drugs are more
quickly released from w/o emulsions |
5. |
They are preferred for formulations meant for internal use as bitter
taste of oils can be
masked. |
They are preferred for formulations meant for external use like creams. |
6. |
O/W emulsions give a positive conductivity test as water
is the external phase which is a good
conductor of electricity. |
W/O emulsions go not give a positive
conductivity test as oil is
the external phase which is
a poor conductor of electricity. |
7. |
|
|
Oral Emulsions: Generally o/w emulsions are used for internal use as the
oil is more readily absorbed in a fine state of subdivision through the gastro
intestinal tract and secondly the preparation becomes more palatable when water
forms the continuous phase, as the medicinal oil is enveloped in a thin film of
emulgent which masks the bitter and oily taste of the
drug like liquid paraffin. Orally emulsions are also used to facilitate the
absorption of the oil soluble drugs like vitamins A,D,
E and K.
Example: Liquid Paraffin Oral Emulsion
Liquid Paraffin 500 ml
Methyl cellulose 20
Vanillin
Chloroform 2.5 ml
Benzoic acid solution 20 ml
Saccharin sodium
Purified Water q.s
1000ml
Uses: Laxative. It acts as an emollient purgative
in chronic constipation especially during pregnancy and old age.
Example: Castor oil Emulsion
Castor oil 16 ml
Gum acacia q.s
Water 80 ml
Uses: Purgative
Example: Cod-Liver oil Emulsion
Cod-liver oil 30 ml
Syrup 12 ml
Ferric ammonium citrate
Cinnamon water q.s. 90
ml
Uses: Source of vitamin A and D. It is used as
dietary supplement in infants and children to prevent the occurance
of rickets and to improve nutrition in undernourished children and patients
with rickets.
Rectal Emulsions: Enemas are formulated as o/w
emulsions.
Topical Emulsions: For external use, emulsions may
be either o/w or w/o type. Emulsions finds the maximum use in topical preparations , both for therapeutic and cosmetic use.
Therapeutically they are used as carrier for a drug. In cosmetic industry o/w
emulsions have been used for formulation of moisturing
lotions, hand lotions and make up foundation lotions. When oily layers are
desired to prevent moisture loss from the surface of skin, for barrier action
and for cleansing action, then w/o emulsions are formulated like cold creams.
Example: Antiseptic cream
Cetrimide 1g
Cetostearyl alcohol
White soft paraffin
Liquid paraffin
Purified water
Uses: Antiseptic cream for the treatment of cuts, wounds and burns.
Example: Cold Cream
Liquid paraffin
Hard paraffin
Lanette wax
Glycerine
Water
Propyl paraben
Uses: Skin protective and skin smoothner.
EMULSIFYING AGENTS
These are the
substances added to an emulsion to prevent the coalescence of the globules of
the dispersed phase. They are also known as emulgents
or emulsifiers. These agents have both a hydrophilic and a lipophilic
part in their chemical structure. All emulsifying agents concentrate at and are
adsorbed onto the oil/water interface to provide a protective barrier around
the dispersed droplets. In addition to this protective barrier, emulsifiers
stabilize the emulsion by reducing the interfacial tension of the system. Some
agents enhance stability by imparting a charge on the droplet surface thus
reducing the physical contact between the droplets and decreasing the potential
for coalescence. Thus these act in three ways:
1) Formation of a
protective barrier
2) Reduction of
interfacial tension
3) Decreasing the
potential for coalescence by forming an electrical double layer
Interfacial phenomena
Interfacial
phenomena occurs at the limit between
two immiscible phases, so-called surface or interface. When one phase is
fragmented into (small) pieces which are dispersed in another (continuous)
phase, a so-called dispersion or dispersed system is produced.
Emulsifying agents
have two fundamental properties. On one hand they tend to be located
preferentially at the interface between a polar and a nonpolar
phase. The phenomenon according to which a molecule comes from the bulk of a
solution to place itself at the interface (with some specific orientation) is
called adsorption, and is characteristic of many amphiphilic
molecules. On the other hand surfactant molecules in solution exhibit a
tendency to self associate to produce aggregation polymer called micelles, as
well as other structures.
All the properties of
surfactant solutions come from one of these fundamental properties.
Amphiphile
An amphiphile
is a chemical substance that possesses some affinity for both the polar
substances and the apolar ones. Generally speaking
these affinities are referred to as hydrophilic and lipophilic
(or hydrophobic) respectively since the polar solvents are in most cases
aqueous solutions and the apolar phases are organic
"oils".
Most amphiphilic substances are surfactants, i.e., substances
that are preferentially located at a surface or interface, where the polarity
changes drastically within a few angstroms of distance.
Adsorption
When a surfactant
molecule goes to the interface and locates itself there with some preferential
orientation, it is said that the molecule is adsorbed. Adsorption is a
spontaneous phenomenon which is driven by a reduction of the energy when the
surfactant lyophobic group is removed from the
solvent, and when one or both affinities are satisfied respectively at a
surface or at an interface.
Adsorption is a
dynamic phenomenon which is opposed by desorption,
i.e., the transfer of a surfactant molecule to a bulk phase. The adsorption and
desorption steps are often very rapid; as a
consequence an adsorption-desorption equilibrium is
reached after some time, which depends upon the surfactant concentration in the
bulk phase.
Since the surfactant
molecule has a lower free energy when it is adsorbed at interface than in the
solvent bulk phase, the equilibrium is very much displaced toward the adsorbed
state. In fact the interface is very rapidly covered by a monolayer of
surfactant molecules. In such monolayer the molecules are arranged in some
specific pattern which depends upon structural and geometrical characteristics.
Self-Association
The second fundamental
property of surfactant molecules is their capability of self-association in
aqueous or non aqueous solutions.
The tendency of the
surfactant molecules to associate depends upon the formation of an adsorbed
monolayer, which is the first step of surfactant association.
When the surfactant
concentration increases in the aqueous phase, the surfactant molecules first
saturate the interface, and then accumulate in the solution. Each time a new
surfactant molecule is added to the solution, the unfavorable interaction
between the surfactant hydrophobic tail and the water molecules is increased.
At some point the surfactant molecules start aggregating into the so-called
micelles, a self-association structure in which the hydrophobic tail is removed
from the aqueous environment. The concentration at which the first micelles are
formed is called the Critical Micelle Concentration, which is abbreviated as
CMC. The CMC is the concentration at which the factors which favor the
formation of the micelle (for instance the hydrophobic effect) start dominating
the effects which oppose it.
Micellar solutions are able to solubilize
different kinds of substance, and this capacity of solubilization
is one of the most important properties of the surfactant solutions.
Hydrophobic substances, i.e., oils, can be solubilized
inside the micelles core, sometimes in very sizeable amounts. Some extreme
cases are known in which the solubilized oil volume
is actually larger than the aqueous solvent volume; for such situation to
happen, the solution must contain a very large number of micelles and the
micelles must be considerably swollen. These micelles are no longer spherical,
but cigar shaped or hexagonally packed or even degraded into lamellar liquid
crystals.
The choice of
selection of emulsifying agent plays a very important role in the formulation
of a stable emulsion. No single emulsifying agent possesses all the properties
required for the formulation of a stable emulsion therefore sometimes blends of
emulsifying agents have to be taken.
CRITERIA FOR THE
SELECTION OF EMULSIFYING AGENTS
An ideal emulsifying
agent should posses the following characteristics:
It should be able to
reduce the interfacial tension between the two immiscible liquids. It should be
physically and chemically stable, inert and compatible with the other ingredients
of the formulation. It should be completely non irritant and non toxic in the
concentrations used. It should be organoleptically
inert i.e. should not impart any colour, odour or taste to the preparation. It should be able to
form a coherent film around the globules of the dispersed phase and should
prevent the coalescence of the droplets of the dispersed phase. It should be
able to produce and maintain the required viscosity of the preparation.
CLASSIFICATION OF
EMULSIFYING AGENTS
Emulsifying agents can
be classified as:
1. Natural Emulsifying
agents: A large number of emulsifiers are natural products derived from plant
or animal tissue. Most of the emulsifiers form hydrated lyophilic
colloids (called hydrocolloids) that form multimolecular
layers around emulsion droplets. Hydrocolloid type emulsifiers have little or
no effect on interfacial tension, but exert a protective colloid effect,
reducing the potential for coalescence, by:
· providing a
protective sheath around the droplets
· imparting a charge
to the dispersed droplets (so that they repel each other)
· swelling to increase
the viscosity of the system (so that droplets are less likely to merge)
Natural emulsifying
agents from vegetable sources: These consist of agents which
are carbohydrates and include gums and mucilaginous substances. Since these
substances are of variable chemical composition, these exhibit considerable
variation in emulsifying properties. They are anionic in nature and produce o/w
emulsions. They act as primary emulsifying agents as well as secondary
emulsifying agents (emulsion stabilizers). Since carbohydrates acts a good
medium for the growth of microorganism, therefore emulsions prepared using
these emulsifying agents have to be suitable preserved in order to prevent microbial
contamination. E.g. tragacanth, acacia, agar, chondrus (Irish Moss), pectin and
starch.
Gum acacia
It is generally used
in the concentration of 8-15% and gives a stable and palatable emulsion over
the pH range of 2-10. Emulsions tend to cream using this as the viscosity is
low.
Tragacanth
Used in the
concentration range of 1-2%. Rarely used now because it forms thick and coarse
emulsions.
Agar
Used in the
concentration of 2%.
Starch
Used
in concentration of 2-5%.
Rarely used because it forms coarse emulsions.
Pectin
Used as 1%. Acts as emulsion stabiliserin acacia
emulsion.
Natural emulsifying
agents from animal source: The examples include gelatin, egg yolk and wool fat
(anhydrous lanolin). Type A gelatin (Cationic) is
generally used for preparing o/w emulsion while type B gelatin is used for o/w
emulsions of pH 8 and above. Lecithin and cholesterol present in egg yolk also
act as emulsifying agent. They show surface activity and are used for
formulating o/w emulsions. However they are used only for extemporaneous
preparation and not for commercial preparation as it darken and degrade rapidly
in unpreserved systems. Wool fat is mainly used in w/o emulsions meant for
external use. They absorb large quantities of water and form stable w/o emulsions
with other oils and fats. Animal derivatives are more likely to cause allergic
reactions and are subject to microbial growth and rancidity. Their advantage is
their ability to support formation of w/o creams.
Gelatin
Used
in concentration of 1%. Two
grades are available-Pharmagol A which has an acidic
pH and Pharmagol B, which has an alkaline pH.
Emulsions prepared have agreeable taste but are prone to bacterial
contamination. Gelatin is used for
preparing o/w emulsions.
Egg yolk
It contains lecithin which
is a phospholipids acting as an emulsifier.
Used in concentration range of 12-15%. Mainly used for extemporaneous
preparation as long term stability is a problem. It forms stable o/w emulsions, which needs to
be refrigerated to prolong shelf life.
2. Semi-synthetic
polysaccharides: Includes mainly cellulose derivatives like sodium carboxy methyl cellulose, hydroxyl propyl
cellulose and methyl cellulose. They are used for formulating o/w type of
emulsions. They are nontoxic, and are less subject to microbial growth. They
primarily act by increasing the viscosity of the system. e.g.,
methyl cellulose, hydroxypropyl cellulose and sodium carboxy methyl cellulose.
Methyl cellulose
This is non ionic in
nature and is stable over a wide pH range. It is mainly used for emulsification
of mineral and vegetables oil. Drawback is that it gets precipitated in the
presence of large quantities of electrolytes.
Sodium carboxymethyl cellulose
It is anionic in
nature. Acts as an true emulsifier and emulsion
stabilizer.
3. Synthetic
emulsifying agents: This group contains surface active agents which act by
getting adsorbed at the oil water interface in such a way that the hydrophilic
polar groups are oriented towards water and lipophillic
non polar groups are oriented towards oil, thus forming a stable film. This
film acts as a mechanical barrier and prevents coalescence of the globules of
the dispersed phase. They are classified according to the ionic charge
possessed by the molecules of the surfactant e.g., anionic, cationic, non-ionic
and ampholytic.
Anionic Surfactants:
The long anion chain on dissociation imparts surface
activity, while the cation is
inactive. These agents are primarily used for
external preparations and not for internal use as they have
an unpleasant bitter
taste and irritant action on the intestinal mucosa.
e.g., alkali soaps, amine soaps,
metallic soaps, alkyl sulphates
and phosphates and alkyl sulphonates.
Alkali soaps
Produce good oil in
water emulsions. Unstable at pH below 10 and are incompatible with acids and
polyvalent inorganic and long chain organic cations.
Amine soaps
Used for preparing o/w
emulsions. Stable to the presence of calcium ions or changes in pH
Bold text Metallic
soaps
Generally used for
formulating w/o emulsions. They are usually insoluble in water
Alkyl sulphates and phosphates
They form o/w
emulsions.
Alkyl sulponates
Used
mainly as wetting agents and form stable o/w emulsions along with a secondary
emulsifying agent.
Cationic surfactants:
The positive charge cations produced on dissociation
are responsible for emulsifying properties. They are mainly used in external
preparations such as lotions and creams. Quaternary ammonium compounds such as cetrimide, benzalkonium chloride
and benzethonium chloride are examples of important
cationic surfactants. These compounds besides having good antibacterial
activity are also used in combination with secondary emulsifying agents to
produce o/w emulsions for external application.
Non-ionic surfactants:
They are the class of surfactants widely used as emulsifying agents. They are
extensively used to produce both oil in water and water in
oil emulsions for internal as well as external use. The emulsions
prepared using these surfactants remain stable over a wide range of pH changes
and are not affected by the addition of acids and electrolytes. They also show
low irritancy as compared to other surfactants. E.g. glyceryl esters such as glyceryl monostearate, propylene glycol monostearate,
macrogol esters such as polyoxyl
stearates and polyoxyl-castor
oil derivatives, sorbitan fatty acid esters such as
spans and their polyoxyethylene derivatives such as tweens (polysorbates).
Ampholytic surfactants: These are the substances whose ionic
charge depends on the pH of the system. Below a certain pH, these are cationic
while above a defined pH, these are cationic. At intermediate pH these behave
as zwitterions. e.g. lecithin.
4. Finely Divided
Solids: This group consists of finely divided solids having balanced
hydrophilic lipophillic properties. They accumulate
at the oil/water interface and form a coherent interfacial film around the
droplets of dispersed phase globules and prevent coalescence. If the solid particles are preferentially wetted by oil, a w/o
emulsion is formed while if wetting is done by water then o/w emulsion is seen.
e.g., bentonite, aluminium magnesium stearate, attapulgite, colloidal anhydrous silica and hectorite. The emulsions formed using finely divided
solids are stable and less prone to microbial contamination.
5. Auxillary
Emulsifying Agents
A variety of fatty
acids (e.g., stearic acid), fatty alcohols (e.g., stearyl or cetyl alcohol), and
fatty esters (e.g., glyceryl monostearate)
serve to stabilize creams through their ability to thicken the emulsion.
Because these agents have only weak emulsifying properties, they are always
used in combination with other emulsifiers.
Selection of
Emulsifying Agents using HLB method
A system was developed
in 1949 by William C. Griffin to assist making systemic decisions about the
amounts and types of surfactants needed in stable products. The system is
called the HLB (hydrophile-lipophile balance) system
and has an arbitrary scale of 1 - 18. HLB numbers are experimentally determined
for the different emulsifiers.
HLB
RANGE USE
0-3 Antifoaming agents
4-6 W/O emulsifying
agent
7-9 Wetting agents
8-18 O/W emulsifying
agent
13-15 Detergents
10-18 Solubilizing agents
An emulsifier having a
low HLB number indicates that the number of hydrophilic groups present in the
molecule is less and it has a lipophillic character.
For example, spans generally have low HLB number and they are also oil soluble.
Because of their oil soluble character, spans cause the oil phase to
predominate and form a w/o emulsion.
A higher HLB number
indicate that the emulsifier has a large number of hydrophilic groups on the
molecule and therefore is more hydrophilic in character. Tweens
have higher HLB numbers and they are also water soluble. Because of their water
soluble character, tweens will cause the water phase
to predominate and form an o/w emulsion.
DISADVANTAGE OF THE
HLB SYSTEM
It does not take into
account:
- the
effect of temperature
- the
presence of additives
- the
concentration of emulsifier
HLB values of some
common emulsifying agents
Emulsifying Agent : HLB Value
Acacia: 8
Polysorbate 20 (Tween 20): 16.7
Polysorbate 60: (Tween 60): 14.9
Polysorbate 80 (Tween 80): 15
Oleic acid: 4.3
Sorbitan monolaurate (Span 20):
8.6
Sorbitan monolaurate (Span 60):
4.7
Sorbitan monolaurate (Span 80):
4.3
Since emulsion (o/w or w/o) looks the same in appearance with naked eyes,
therefore certain tests have been developed to differentiate between them. At
least two tests should be done to reach a conclusive decision about the
identity of the emulsion.
In this test the emulsion is diluted either with
oil or water. If the emulsion is o/w type and it is diluted with water, it
will remain stable as water is the dispersion medium but if it is diluted with
oil, the emulsion will break as oil and water are not miscible with each other.
Oil in water emulsion can easily be diluted with an aqueous solvent whereas
water in oil emulsion can be diluted with a oily
liquid.
Fig: Dilution Testfor
oil in water emulsion
Fig: Dilution test for water in oil emulsion
This test is based on the basic principle that
water is a good conductor of electricity. Therefore in case of o/w emulsion , this test will be positive
as water is the external phase. In this test. An
assembly consisting of a pair of electrodes connected to a lamp is dipped into
an emulsion. If the emulsion is o/w type, the lamp glows.
Fig: Conductivity test for oil in water emulsion
Fig: Conductivity test for water in oil emulsion
In this test, when an emulsion is mixed with a
water soluble dye such as amaranth and observed under the microscope, if the continuous phase appears red, then it means
that the emulsion is o/w type as water is the external phase and the dye will
dissolve in it to give color but if the scattered globules appear red and
continuous phase colorless, then it is w/o type. Similarly if an oil soluble
dye such as Scarlet red C or Sudan III is added to an emulsion and the
continuous phase appears red, then it w/o emulsion.
Fig: Dye solubility test
When a filter paper soaked in cobalt chloride
solution is added to an emulsion and dried, it turns from blue to pink,
indicating that the emulsion is o/w type.
If an emulsion on exposure to ultra-violet
radiations shows continuous florescence under microscope, then it is w/o type and if it shows only spotty
fluorescence, then it is Oil in o/w type.
An emulsion is a thermodynamically unstable
preparation so care has to be taken that the chemical as well as the physical
stability of the preparation remains intact throughout the shelf life. There
should be no appreciable change in the mean particle size or the size
distribution of the droplets of the dispersed phase and secondly droplets of
the dispersed phase should remain uniformly distributed throughout the system.
Instabilities seen in emulsion can be grouped as
An emulsion is said to cream when the oil or fat
rises to the surface, but remains in the form of globules, which may be
redistributed throughout the dispersion medium by shaking. An oil of low
viscosity tends to cream more readily than one of high viscosity. Increasing
the viscosity of the medium decreases the tendency to cream. Creaming is a
reversible phenomenon which can be corrected by mild shaking. The factors
affecting creaming are best described by strokeâ™s law
V= 2r2 (d1-d2) g/9η
Where V= rate of creaming
r=radius of globules
d1= density of dispersed phase
d2= density of dispersion medium
g= gravitational constant
η = viscosity of the dispersion medium
The following approaches can be used for
decreasing Creaming
Reduction of globule size: According to strokeâ™s
law, rate of creaming is directly proportional to the size of globules. Bigger
is the size of the globules, more will be the creaming. Therefore in order to
minimize creaming, globule size should be reduced by homogenization.
Increasing the viscosity of the
continuous phase: Rate of creaming is inversely proportional to the viscosity of the
continuous phase i.e. more the viscosity of the continuous phase, less will the
problem of creaming. Therefore to avoid creaming in emulsions, the viscosity
of the continuous phase should be increased by adding suitable viscosity
enhancers like gum acacia, tragacanth etc.
Occasionally, it happens that an emulsion cracks
during preparation, i.e., the primary emulsion does not become white but
acquires an oily translucent appearance. In such a case, it is impossible to
dilute the emulsion nucleus with water and the oil separates out. Cracking of
emulsion can be due to addition of an incompatible emulsifying agent, chemical
or microbial decomposition of emulsifying agent, addition of electrolytes,
exposure to increased or reduced temperature or change in pH.
In phase inversion o/w type emulsion changes into
w/o type and vice versa. It is a physical instability. It may be brought about
by the addition of an electrolyte or by changing the phase volume ratio or by
temperature changes. Phase inversion can be minimized by using the proper
emulsifying agent in adequate concentration, keeping the concentration of
dispersed phase between 30 to 60 % and by storing the emulsion in a cool place.
Stability of the active ingredient
Stability of the excipients
Visual appearance
Color
Odor (development of pungent odor/loss of
fragrance)
Viscosity, extrudability
Loss of water and other volatile vehicle
components
Concentration of emulsifier
Order of addition of ingredients
Particle size distribution of dispersed phases
pH
Temperature of emulsification
Type of equipment
Method and rate of cooling
Texture, feel upon application (stiffness,
grittiness, greasiness, tackiness, spreadibility)
Microbial contamination/sterility (in the unopened
container and under conditions of use)
Release/bioavailability (percutaneous
absorption)
Phase distribution, Phase Inversion
(homogeneity/phase separation, bleeding
Depending on the use, emulsions should be packed
in suitable containers. Emulsions meant for oral use are usually packed in well
filled bottles having an air tight closure. Light sensitive products are packed
in amber coloured bottles. For viscous emulsions,
wide mouth bottles should be used. The label on the emulsion should mention
that these products have to be shaken thoroughly before use. External use
products should clearly mention on their label that they are meant for external
use only. Emulsions should be stored in a cool place but refrigeration should
be avoided as this low temperature can adversely effect
the stability of preparation.
Preservation from microorganisms:
It is necessary to preserve the emulsions from microorganisms as these can proliferate easily
in emulsified systems with high water content, particularly if carbohydrates, proteins or steroidal materials are also present.
Contamination due to microorganisms can result in
problems such as color and odor change, gas production, hydrolysis, pH change
and eventually breaking of emulsion. Therefore is necessary that emulsified
systems be adequately preserved. An ideal preservative should be nonirritant, nonsensitizing and nontoxic in the concentration used. It
should be physically as well as chemically compatible with other ingredients of
the emulsions and with the proposed container of the product. It should not
impart any taste, color or odor to the product. It should be stable and
effective over a wide range of pH and temperature. It should have have a wide spectrum of activity
against a range of bacteria, yeasts and moulds. The selective preservative
should have high water solubility and a low oil/water partition coefficient. It
should have bactericidal rather than bacteriostatic
activity.
Examples of antimicrobial preservatives used to
preserve emulsified systems include parahydroxybenzoate
esters such as methyl, propyl and butyl parabens, organic acids such as ascorbic acid and benzoic acid, organic mercurials
such as phenylmercuric acetate and phenylmercuric nitrate, quarternary
ammonium compounds such as cetrimide, cresol
derivatives such as chlorocresol and miscellaneous
agents such as sodium benzoate, chloroform and phenoxyethanol.
Preservation from oxidation:
Oxidative changes such as rancidity and spoilage
due to atmospheric oxygen and effects of enzymes produced by micro-organisms is
seen in many emulsions containing vegetables and mineral oils and animal fats. Antioxidants can be used to prevent the changes
occurring due to atmospheric oxygen.
Antioxidants are agents having a high affinity for
oxygen and compete for it with labile substances in the formulation. The ideal
antioxidant should be nontoxic, nonirritant, effective at low concentration
under the expected conditions of storage and use, soluble in the medium and
stable. Antioxidants for use in oral preparation should also be odorless and
tasteless.
Some of the commonly used antixidants
for emulsified systems include alkyl gallate such as
ethyl, propyl or dodecyl gallate, butylated sshydroxyanisole (BHT), butylated
hydroxytoluene (BHT)
Preparation Of Emulsions
Preparation of emulsions depends on the scale at which it is produced. On
small scale mortar and pestle can be used but its efficiency is limited. To
overcome these drawback small electric mixers can be used although care must be
exercised to avoid excessive entrapment of air. For large scale production
mechanical stirrers are used to provide controlled agitation and shearing
stress to produce stable emulsions.
Methods for preparing Emulsions for Internal use
The methods commonly used to prepare emulsions can be divided into two categories:
Trituration Method
This method consists of dry gum method and wet gum method.
Dry Gum Method
In this method the oil is first triturated with gum with a little amount of
water to form the primary emulsion. The trituration
is continued till a characteristic ‘clicking’ sound is heard and a thick white
cream is formed. Once the primary emulsion is formed, the remaining quantity of
water is slowly added to form the final emulsion.
Wet Gum Method
As the name implies, in this method first gum and water are triturated
together to form a mucilage. The required quantity of oil is then added
gradually in small proportions with thorough trituration
to form the primary emulsion. Once the primary emulsion has been formed
remaining quantity of water is added to make the final emulsion.
Bottle Method
This method is employed for preparing emulsions containing volatile and
other non-viscous oils. Both dry gum and wet gum methods can be employed for
the preparation. As volatile oils have a low viscosity as compared to fixed
oils, they require comparatively large quantity of gum for emulsification. In
this method, oil or water is first shaken thoroughly and vigorously with the
calculated amount if gum. Once this has emulsified completely, the second
liquid (either oil or water) is then added all at once and the bottle is again
shaken vigorously to form the primary emulsion. More of water is added in small
portions with constant agitation after each addition to produce the final
volume.
Proportions of. |
|||
Type of Oil |
Oil |
Water |
Gum |
Fixed Oil |
4 |
2 |
1 |
Mineral Oil |
3 |
2 |
1 |
Volatile Oil |
2 |
2 |
1 |
Table: 1 Proportions of Oil, Water and Gum required for formation of
primary emulsion
Methods for preparing Emulsions for External use:
Emulsions meant for external application such as creams, lotions and
liniments contain in their formula waxy solids which require melting before
mixing. Such emulsions may be prepared by melting the oily components
separately at 60
PROPERTIES OF EMULSIONS The basic properties which
should be present in an emulsion include appearance, feel, odour,
desirable viscosity, consistency, effectiveness and stability. These properties
depends on the ingredients, type of emulsion, ratio of the two phases, type and
quantity of emulsifying agents and method of emulsification .
O/w emulsions will generally have a sheen or matte surface as compared to w/o
emulsions which have a shiny or oily surface due to the presence of oil as
external phase. W/o emulsions are oily and greasy in nature,
not easily removable from the surface of the skin whereas o/w emulsions are non
greasy and easily removable from the skin surface. The viscosity of the
emulsions depends generally on the viscosity of the continuous phase. As the
ratio of dispersed phase increases, the viscosity also increases to a point
where emulsion starts loosing its fluidity.
The following are the quality control tests done
for emulsions:
1. Determination of particle size and particle
count:
Determination of changes in the average particle size or the size distribution
of droplets is an important parameter used for the evaluation of emulsions. It
is performed by optical microscopy, sedimentation by using Andreasen
apparatus and Coulter counter apparatus.
2. Determination of viscosity: Determination of
viscosity is done to assess the changes that might take place during aging.
Emulsions exhibit non-newtonian type of flow characterstics. The viscometers which should be used
include cone and plate viscometers. Capillary and falling sphere type of viscometrs should be avoided. For viscous emulsions, the
use of penetrometer is recommended as it helps in the
determination of viscosity with age. In case of o/w emulsions, flocculation of
globules causes an immediate increase in viscosity. After this change, the
consistency of the emulsion changes with time. In case of w/o emulsions, , the dispersed phase particles flocculate quite rapidly
resulting in a decrease in viscosity, which stabilizes after 5 to 15 days. As a
rule, a decrease in viscosity with age reflects an increase of particle size
due to coalescence.
3. Determination of phase separation: This is another
parameter used for assessing the stability of the formulation. Phase separation
may be observed visually or by measuring the volume of the separated phases.
4. Determination of electrophoretic
properties: Determination of electrophoretic properties like
zeta potential is useful for assessing flocculation since electrical charges on
particles influence the rate of flocculation. O/W emulsion having a fine
particle size will exhibit low resistance but if the particle size increase,
then it indicates a sign of oil droplet aggregation and instability.
Stability of emulsions is an important parameter for the formulator. Stability
testing of emulsions involves determining stability at long term storage
conditions, accelerated storage conditions, freezing and thawing conditions.
Stress conditions are applied in order to speed up the stability testing. The
stress conditions used for speeding up instability of emulsions include:
Centrifugal force Agitational
force Aging and temperature The following physical
parameters are evaluated to assess the effect of any of the above stress
conditions:
-
· Phase separation
-
· Viscosity
-
· Electrophoretic properties
-
· Particle size and particle count
Particle size and size distribution The freeze-thaw cycling
technique used to assess emulsions for stress testing for stability testing
result in increase of particle growth and may indicate
future state after long storage. It is of importance to study the changes for
absolute particle size and particle size distribution. It is performed by
optical microscopy, sedimentation by using Andreasen
apparatus and Coulter counter apparatus. None of these methods are direct
methods. However microscopic method allows the observer to view the actual
particles.
Rheological studies Cone and Plate
viscometer with variable shear stress control can be used for evaluating
viscosity of emulsions.
5.
Formulation of emulsions
Calculation of the components’ amount of a
primary emulsion
The oil’s amount is indicated in the prescription. If
there are no indications, then take:
The amount of the emulsifier is determined by its
ability to emulsify
The amount of water to form a primary emulsion is determined by the emulsifier’s dissolution in water
To 10.0 g of the oil add:
Emulsifier |
Water to prepare the primary emulsion |
5.0 of gelatose |
7.5 ml of water |
2.0 of Tween-80 |
2-3 ml of water |
1.0 of methyl cellulose |
as 5 % solution - 20.0 |
0.5 g of sodium carboxymethylcellulose |
as 5 % solution – 10.0 |
5.0 g of starch |
as 10 % solution – 50.0 |
Addition of medicinal substances (MSs) to
emulsions
MSs soluble in water (They can be used as concentrated
solutions.) dissolve in the portion of water
intended for dilution of the
primary emulsion. Similarly add
alcoholic solutions, syrups, extracts
MSs soluble in oil dissolve in oil before introducing it into the primary emulsion The
amount of an emulsifier is calculated by the weight of the oily solution. (exception - phenylsalycilate (the oily solution does not have
the antiseptic action)
MSs insoluble in water
and oil àccording to the
rules of suspension’s preparation, add them as a fine powder thoroughly
triturating with the emulsion prepared. If necessary, a stabilizer is
added
WCP (reverse side)
(as an emulsifier – solution of Methylcellulose 5 %) Peach oil: 100.0 / 10 = 10.0 Solution of ÌÑ 5 %: 10.0 õ 2 = 20.0 |
Place
Since
the emulsion contains caffeine benzoate sodium, approximately 20-25 ml of the
purified water (or 10 % concentrated solution - 5 ml is used) for its
dissolution is left. Then dilute the primary emulsion with the
remaining amount of water and add the solution of caffeine benzoate sodium.
WCP
(front side)
Date
¹
Pr.
Sol. Methylcellulosae 5 % 20.0 |
WCP (reverse side)
(as
an emulsifier – Tween-80) Peach oil: 200.0 / 10 = 20.0 Oil phase: 20.0 + 1.0 = 21.0 Tween-80: 21.0 / 5 = 4.2 x »
5 ml |
Weigh out
Rp.: Benzylii
benzoatis 20.0
Saponis viridis
2.0
Aquae purificatae 78 ml
Misce. Da. Signa. Apply on the hands.
To prepare this emulsion replace
WCP
(front side)
Date
¹
Pr.
Olei Ricini 20.0
Camphorae 1.0
Tweeni-80 4.2
Aquae purificatae 75.8 ml
mtotal =
201.0
Prepared
by: (signature)
Checked by: (signature)
Melt
Stratification of the emulsion is possible in 4 days after preparation;
it is easily restored when shaking
Emulsion is stable for 2 months.