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Vibrio.

June 13, 2024

Vibrio. Laboratory diagnosis

The Cholera Vibrio. The causative agents of cholera are the classical Vibrio cholera biovars discovered by R. Koch in 1883 and the El Tor vibrio biovar isolated from the cadaver of a pilgrim on the Sinai peninsula by Gotschlich in 1906, RO- and O139 strains.

Vibrio cholerae biovar Proteus (N. Gamaleya, 1888) and Vibrio cholerae biovar albensis were discovered” later. V. cholerae was described by F. Pacini in 1854.

Morphology. Cholera vibrios are shaped like a comma or a curved rod measuring 1-5 mcm in length and 0.3 mcm in breadth (Fig.).

Figure. Vibrio cholerae: 1-pure culture; 2- flagellate vibrios

They are very actively motile, monotrichous, nonsporeforming, noncapsulated, and Gram-negative.

The cholera vibrio is subject to individual variation when it is exposed to physical and chemical factors. On artificial media and in old cultures it occurs in the form of grains, globes, rods, threads, clubs or spirals. When it is re-inoculated into fresh media, the organism assumes its initial form.

Electron microscopy reveals flagella 25 nm thick and three-layer cell wall and cytoplasmatic membrane. Small vacuoles are seen between the wall and the cytoplasmatic membrane. The formation of the vacuoles is assumed to be linked with exotoxin synthesis. The nucleoid is in the centre of the cell. No essential difference has beeoted between the structure of the classical cholera vibrion and that of the El Tor vibrio. The G-C content in DNA ranges between 45 and 49 per cent.

Cultivation. Cholera vibrios are facultative (anaerobes). The optimum growth temperature is 37° C, and growth is arrested below 14 °C and above 42° C. The organisms grow readily on alkaline media at pH 6.0-9.0, and on solid media the colonies are transparent with a light-blue hue, forming domes with smooth edges. On gelatin the organisms produce transparent granular colonies which, when examined under a microscope, resemble broken glass. In 48 hours the medium surrounding the colonies becomes liquefied and the colonies sink into this area. Six-hour-old cultures on alkaline meat broth and peptone water produce a pellicle, which consists of cholera vibrios.

The organism is also subjected to cultural changes. It dissociates from the S-form to the R-form, this process being accompanied by profound changes in antigenic structure.

Fermentative properties. The cholera vibrio liquefies coagulated serum and gelatin; it forms indole and ammonia, reduces nitrates to nitrites, breaks down urea, ferments glucose, levulose, galactose, maltose, saccharose, mannose, mannite, starch, and glycerine (slowly) with acid formation but does not ferment lactose in the first 48 hours, and always coagulates milk. The cholera vibrio possesses lysin and ornithine decarboxylases and oxidase activity. B. Heiberg differentiated vibrios into biochemical types according to their property of fermenting mannose, arabinose, and saccharose. Eight groups of vibrios are known to date; the cholera vibrios of the cholerae and El Tor biovar belong to biochemical variant 1.

The haemolytic activity and haemagglutinating properties of the cholera vibrios in relation to different erythrocytes (sheep, goat, chick, and others) as well as the ability for forming acetylmethylcarbinol are not stable characteristics and are taken into account as less important data in differentiating microbes of the genus Vibrio.

Toxin production. The cholera vibrio produces an exotoxin (cholerogen) which is marked by an enterotoxic effect and plays an important role in the pathogenesis of cholera; the endotoxin also exerts a powerful toxic effect. The cholera vibrios produce fibrinolysin, hyaluronidase, collagenase, mucinase, lecithinase, neuraminidase, and proteinases.

V cholerae produces diarrhea as a result of the secretion of an enterotoxin, choleratoxin, which acts identically to E coli LT to stimulate the activity of the enzyme adenylcyclase. This, in turn, converts ATP to cAMP, which stimulates the secretion of Cl^– and inhibits the absorption of NaCl. The copious fluid that is lost also contains large amounts of bicarbonate and K⁺. Thus, the patient has both a severe fluid loss and an electrolyte imbalance.

The enterotoxin has been shown to bind specifically to a membrane ganglioside designated GM₁. Interestingly, V cholerae produces a neuraminidase that is unable to remove the y-acetylneuraminic acid from GM₁, but it is able to convert other gangliosides to GM₁, thus synthesizing even more receptor sites to which its enterotoxin can bind. Like the LT of E coli, choleragen is composed of five B subunits that react with the cell receptor, an Ai-active subunit that enters the cell and, together with a cellular ADP-ribosylating factor, carries out the ADP-ribosylation of the GTP-binding protein, and a small A; subunit that seems to link the Ai subunit to the B subunit. Interestingly, unlike LT, the DNA en-coding choleragen is not plasmid mediated but is on the chromosome of V cholerae.

CT (as well as the LT produced by E coli) can be quantitated by a number of in vivo, cell culture, or immunologic assay units. In one method, a segment of rabbit small intestine is tied to form a loop. Enterotoxin is serially diluted, and an aliquot of each dilution is injected into a loop. The highest dilution that stimulates fluid accumulation in the loop is recorded as the titer of the enterotoxin. A second method takes advantage of the fact that cAMP causes a morphologic response in cultured Chinese hamster ovary cells, and that enterotoxin will induce such cells to produce cAMP. To quantitate enterotoxin using this assay, a standard, curve is established (with purified enterotoxin) that can be used subsequently to assay an unknown enterotoxin from E coli or V. cholerae (Fig.).

FIGURE. A standard curve to equate Escherichia coli enterotoxin with purified cholera toxin. The percentage of Chinese hamster ovary cells that have elongated after growing 24 hours in the presence of cholera toxin in 1% fetal calf serum is plotted against the concentration of cholera toxin present in the culture. As shown, heated toxin or toxin preincubated with antitoxin (anti-CT) have no effect on the morphologic features of the cells.

As is true with essentially all diarrhea-producing bacteria, V cholerae must specifically colonize the intestinal epithelial cells to produce disease. In this case, however, the pili binding the bacteria to the host cells seem to be under the same regulator as choleragen production and, as a result, are termed toxin-coregulated pili. Mutants unable to bind to intestinal cells are avirulent in spite of their ability to produce choleragen. Moreover, antibody directed to toxin-coregulated pili are protective.

Remember that non-01 and non-0139 strains of V.cholerae also cause a wide spectrum of infections, ranging from mild diarrhea to one indistinguishable from classic cholera. Some of these serotypes are known to produce a choleratoxin that is identical to that of the classic biotypes, whereas other products a heat-stable enterotoxin analogous to the ST of E. coli.

Antigenic structure. The cholera vibrios have thermostable O-antigens (somatic) and thermolabile H-antigens (flagellar). The O-antigen possesses species and type specificity, the H-antigen is common for the genus Vibrio. According to the O-antigen content, the vibrios are separated into subgroups of which there are more than 140. The cholerae vibrios, El Tor biovars and biovars cholera belong to the O-1 subgroup. In the 0-1 subgroup there are three O-antigens (A, B, and C) according to the combination of which three serological variants, Ogawa (AB), Inaba (AC) and an intermediate variant Hikojima (ABC), are distinguished.

Classification. Vibrio cholerae belongs to family Vibrionaceae, genus Vibrio consisting of 5 species. The species Vibrio cholerae is subdivided into four biological variants: biovar cholerae, biovar El Tor, biovar Proteus, and biovar albensis.

Biovar cholerae and biovar El Tor of Vibrio cholerae are the causative agents of human cholera. Biovar Proteus of Vibrio cholerae causes diarrhoea in birds and gastroenteritis in humans; biovar albensis of Vibrio cholerae was revealed in fresh water and in human faeces and bile.

Resistance. The cholera vibrio survives for a long time at low temperatures. It lives in faeces for up to a month, in oysters, crabs, on the surface offish and in their intestines from 1 to 40 days, in water for several days, on foodstuffs from 1 to 10 days, and in the intestines of flies from 4 to 5 days. .

The El Tor vibrio is marked by high resistance. It lives more than four weeks in sea and river water, 1-10 days on foodstuff’s, and 4-5 days in the guts of flies. It is possible that under favourable conditions El Tor vibrio may reproduce in various water reservoirs.

The organism shows a low resistance to sunlight, X-rays, desiccation, and high temperatures. It is destroyed instantly at 100°C, and in 5 minutes at 80° C. Cholera vibrios are highly sensitive to disinfectants, particularly to acids (e. g. a 1 :10000 solution of hydrochloric acid kills them within one minute). The organism is also very sensitive to the action of gastric juice.

Pathogenicity for animals. Iature animals are not attacked by cholera, but an intraperitoneal injection of the culture into rabbits and guinea pigs gives rise to general toxicosis and peritonitis which is followed by death.

In his experiments with rabbit-sucklings E. Metchnikoff produced a disease similar to human cholera by oral infection. R. Koch reproduced the disease in guinea pigs previously alkalizing the gastric juice and introducing opium. An intravenous vibrio injection into rabbits and dogs gives rise to lethal toxaemia.

Pathogenesis and diseases in man. Cholera is undoubtedly the most dramatic of the water-borne diseases. As far as is known, cholera was confined to India for the almost 2000 years between its first description by Hindu physicians in 400 b c and its spread to Arabia, Persia, Turkey, and Southern Russia in the early1800s. There were six major pandemics of cholera during the 1800s covering the entire world, killing millions wherever it struck. During one such outbreak in London during 1849, the famous physician, John Snow, traced the spread of the disease to a Broad Street pump from which area residents obtained their water. The spread of cholera in this area was stopped when Snow recommended that the handle of the pump be removed. This is particularly remarkable when one remembers that the germ-theory of disease had not yet been formulated.

The cholera vibrios are transmitted from sick persons and carriers by food, water, flies, and contaminated hands. Via the mouth the organisms gain entrance into the small intestine, where the alkaline medium and an abundance of products of protein catabolism furnish favourable conditions for their multiplication. When the cholera vibrios perish, a large amount of toxin is released. This toxin invades the patient’s blood owing to necrosis of the intestinal epithelium and the resulting condition promotes disturbance of the intestinal vegetative nerve fibres, dehydration of the body, and a development of intoxication.

Cholera is characterized by a short incubation period of several hours to up to 6 days (in a disease caused by the El Tor vibrio it lasts three to five days), and the disease symptoms include general weakness, vomiting, and a frequent loose stool. The stools resemble rice-water and contain enormous numbers of torn-off intestinal epithelial cells and cholera vibrios. The major symptom of cholera is a severe diarrhea in which a patient may lose as much as 10 to 20 L or more of liquid per day. Death, which may occur in as many as 60% of untreated patients, results from severe dehydration and loss of electrolytes.

Three phases can be distinguished in the development of the disease. 1. Cholera enteritis (choleric diarrhoea) which lasts 1 or 2 days. In some cases the infectious process terminates in this period and the patient recovers. 2. Cholera gastroenteritis is the second phase of the disease. Profuse diarrhoea and continuous vomiting lead to dehydration of the patient’s body and this results in lowering of body temperature, decrease in the amount of urine excreted, drastic decrease in the number of mineral and protein substance, and the appearance of convulsions. The presence of cholera vibrios is revealed guite frequently in the vomit and particularly in the stools which have the appearance of rice water. 3. Cholera algid which is characterized by severe symptoms. The skin becomes wrinkled due to the loss of water, cyanosis appears, and the voice becomes husky and is sometimes lost completely. The body temperature falls to 35.5-34° C. As a result of blood concentration cardiac activity is drastically weakened and urination is suppressed.

In severe cases the algid period is followed by the asphyctic phase characterized by cyanosis, dyspnoea, uraemia, azotaemia, and unconsciousness (cholera coma), which lead to prostration and death. Effective treatment and proper nursing care may induce a change of the algid period to the reactive phase during which urination becomes normal, intoxication decreases, and the patient recovers. Fulminate forms of cholera (dry cholera or cholera sicca) may occur in a number of cases.These forms are characterized by the absence of diarrhoea and vomiting and result in death due to severe intoxication. Atypical and latent forms of cholera are exhibited quite frequently, particularly in children, resembling mild cases of gastroenteritis.

Non-specific complications in cholera include pneumonia, erysipelas, phlegmons, abscesses, occasionally sepsis, etc. Among the specific complications cholera typhoid is the most menacing. It is accompanied by a rise in body temperature to 38-39° C, eruptions on the skin, vomiting and fetid loose stools. This condition causes a mortality rate of 80-90 per cent.

Erased and mild forms are observed in 80 to 90 per cent of cases caused by El Tor vibrio. Severe forms with a fatal outcome are encountered in individuals whose condition is aggravated by various somatic diseases which reduce the general body resistance, in those with hypoacidic gastric function, and in elderly persons.

Post-mortem examination of cholera cases reveals distinct hyperaemia of the peritoneum and serosa of the small intestine, which are covered with a sticky exudate. The mucous membrane of the small intestine is congested, peach-coloured, the intestinal epithelium is frequently desquamated, and there are haemorrhages in the submucosa. The vibrios are present in great abundance in the intestinal wall, particularly in Lieberkuhn’s glands, and, not infrequently, in the gall-bladder.

Cholera mortality was quite high in the past (50 to 60 per cent), but has markedly decreased with the application of aetiotropic and pathogenetic therapy. According to WHO, in 1969-1971 it was 17.7 per cent.

Immunity acquired after cholera is high-grade but of short duration and is of an anti-infectious (antibacterial and antitoxic) character. It is associated mainly with the presence of antibodies (lysins, agglutinins, and opsonins). The cholera vibrios rapidly undergo lysis under the influence of immune sera which contain bacteriolysins.

E. Metchnikoff attributed definite significance to phagocytosis following immunity. The normal activity of the stomach, whose contents are bactericidal to the cholera vibrio, plays an essential role in the natural defence mechanism.

Laboratory diagnosis. A strict regimen is established in the laboratory. Examinations are carried out in accordance with the general rules observed for particularly hazardous diseases.

Test specimens are collected from stools, vomit, organs obtained at autopsy, water, objects contaminated by patient’s stools, and, in some cases, from foodstuffs. Certain rules are observed when the material is collected and transported to the laboratory, and examination is made in the following stages.

1. Stool smears stained by a water solution of fuchsin are examined microscopically. In the smears, the cholera vibrios occur in groups similar to shoals of fish (Fig.).

Figure. Vibrio cholerae (stool smear)

2. A stool sample is inoculated into 1 per cent peptone water and alkaline agar. After 6 hours incubation at 37°C the cholera vibrios form a thin pellicle in the peptone water, which adheres to the glass. The pellicle smears are Gram stained, and the culture is examined for motility. A slide agglutination reaction is performed with specific agglutinating 0-serum diluted in a ratio of 1 in 100.

The organisms are then transferred from the peptone water onto alkaline agar for isolation of the pure culture. If the first generation of the vibrios in peptone water is not visible, a drop taken from the surface layer is re-inoculated into another tube of peptone water. In some cases with such re-inoculations, an increase in the number of vibrios is achieved.

The vibrio culture grown on solid media is examined for motility and agglutinable properties. Then it is subcultured on an agar slant to obtain the pure culture.

3. The organism is identified finally by its agglutination reaction with specific 0-serum, determination of its fermentative properties (fermentation of mannose, saccharose, and arabinose), and its susceptibility to phagolysis (Table ).

Table

Differentiation of Biovars of Cholera vibrio

Vibrio

Fermentation within 24 hrs

Seep erythrocyte hemolysis

Lysis by specific O-1 subgroup phages

Agglutination by O-1 cholera serum

Sensitivity to polymixin B

sacharose

mannose

arabinose

Vibrio cholerae biovar cholerae

–

Vibrio cholerae biovar El Tor

–

Vibrio cholerae biovar Proteus

–

Vibrio cholerae biovar albensis

–

Note “A” – carbohydrate fermentation with acid production; “+””– positive result; “–” – negative result; “+” – negative or positive result is not always observed.

The following procedures are undertaken for rapid diagnosis: (1) dark field microscopy of the stool; (2) stool culture by the method of tampons incubated for 16-18 hours in an enrichment medium with repeated dark field microscopy; (3) agglutination reaction by the method of fluorescent antibodies;

(4) bacterial diagnosis by isolation of cholera vibrios (the faecal mass is seeded as a thin layer into a dish containing non-inhibiting nutrient agar and grown for 4-5 hours, the vibrio colonies are detected with a stereoscopic microscope, and the culture is tested by the agglutination reaction with O-serum on glass; (5) since neuraminidase is discharged by the cholera vibrios and enters the intestine, a test for this enzyme is considered expedient as a means of early diagnosis (it is demonstrated in 66-76 per cent of patients, in 50-68 per cent of vibrio carriers, and occasionally in healthy individuals).

Treatment. The mortality rate of cholera can be reduced to less than1% by the adequate replacement of fluids and electrolytes. Antibiotics of the tetracycline group (tetracycline, sigmamycin), amphenicol, and streptomycin are prescribed at first intravenously and then by mouth.

Pathogenetic therapy is very important: control of dehydration, hypoproteinaemia, metabolic disorders, and the consequences of toxicosis, acidosis in particular, by infusion of saline (sodium and potassium) solutions, infusion of plasma or dry serum, glucose, the use of warm bath, administration of drugs which improve the tone of the heart and vessels.

Prophylaxis. Cholera patients and vibrio carriers are the source of the disease. Individuals remain carriers of the El Tor vibrio for a lengthy period of time, for several years. Vibrios of this biotype are widely distributed in countries with a low sanitary level. They survive in water reservoirs for a long time and have been found in the bodies of frogs and oysters. Infection may occur from bathing in contaminated water and fishing for and eating shrimps, oysters, and fish infected with El Tor vibrio.The following measures are applied in a cholera focus:

(1) detection of the first cases with cholera, careful registration of all sick individuals, immediate information of health protection organs;

(2) isolation and hospitalization, according to special rules, of all sick individuals and carriers, observation and laboratory testing of all contacts;

(3) concurrent and final disinfection in departments for cholera patients and in the focus;

(4) protection of sources of water supply, stricter sanitary control over catering establishments, control of flies; in view of the possibility of El Tor vibrio reproducing in water reservoirs under favourable conditions (temperature, the presence of nutrient substrates) systematic bacteriological control over water reservoirs has become necessary, especially in places of mass rest and recreation of the population in the summer;

(5) strict observance of individual hygiene; boiling or proper chlorination of water, decontamination of dishes, hand washing;

(6) specific prophylaxis: immunization with the cholera monovaccine containing 8 thousand million microbial bodies per 1 ml or with the cholera anatoxin. Chemoprophylaxis with oral tetracycline is conducted for persons who were in contact with the sick individual or for patients with suspected cholera.

Immunization with heat-killed cholera organisms seems to give some protection, and recovery from the disease imparts immunity of an unknown degree or duration. Killed whole cells of V cholerae given orally along with purified B subunits of the toxin induced immunity in about 85% of persons who received it. Another experimental engineered oral vaccine consists of a live attenuated V. cholerae El Tor Ogawa strain. This mutant no longer expresses the A subunit of the toxin but does produce B subunits. It seemed to provide good immunity in volunteers but it has not been used in large-scale field trials.

A experimental vaccine that induces toxin-neutralizing antibodies in mice uses. an ingenious technique in which a 45-base-pair oligonucleotide encoding an epitope of the B subunit of CT is inserted into the flagellin gene of an avirulent Salmonella. This 15-amino acid insert was expressed at the flagellar surface without abolishing flagellar function. The concept of placing an immunogen in a prominently displayed position on the bacterial surface could be used as a cholera vaccine as well as for inserting a number of other epitopes from both bacteria and viruses.

Remember, however, that none of these vaccines offer any protection against the newly described 0139 strain of V. cholera, and it is necessary to develop new vaccines for these organisms.

General epidemic measures play the principal role in cholera prophylaxis, whereas immunization is regarded as an auxiliary measure.

Cholera dates back to the most ancient times. Its endemic focus is India (Lower Bengal, and the deltas of the Ganges and Brahmaputra rivers).

There were six cholera pandemics between 1817 and 1926: in 1817-1823, 1826-1837, 1846-1862, 1864-1875, 1883-1896, and in 1900-1926. In 1961-1963 the seventh outbreak of cholera pandemic occurred, it was caused by the El Tor vibrio.

According to WHO, 668650 cholera cases were recorded between 1953 and 1961 in the countries of Asia and Africa and 348752 cases between 1961 and 1966.

Beginning with 1966, over 50 per cent of cholera cases in the countries of Asia were caused by the El Tor vibrio. According to WHO, however, the incidence of cholera induced by the classical Vibrio cholerae has doubled. There were cases with cholera in the southern regions of the Soviet Union (Astrakhan, Odessa, Kerch) in 1970. More than 464307 people sick with cholera were recorded in all countries in the period between 1970 and 1976. Cholera epidemic with high morbidity and
mortality rates occurred in the second half of 1977 in the Near East (Syria, Saudi Arabia, and other countries).

Vibrio Parahaemolyticus. Vibrio parahaemolyticus was discovered in 1963 by R. Sakazaki and colleagues. It was isolated from sea water, sea animals (fish) and the stool of humans sick with acute enteritis. Two biovars have been identified, biovar 1 (parahaemolyticus) and biovar 2 (alginolyticus). According to the 0-antigen, the Vibrio haemolyticus contains 12 serovars.

Vibrio parahaemolyticus is the causative agent of toxinfections. It produces haemolysin which has an enterotoxic effect. The ocean water along the coast of Japan is the natural reservoir of Vibrio parahaemolyticus. The sea fish and crustaceans are seeded with the organism but it reproduces within them only after they have been caught.

Vibrio parahaemolyticus strains isolated from humans cause lysis of erythrocytes and a cytopathic effect in human tissue cell cultures, whereas strains isolated from food and sea water are devoid of these properties. Fermentation of saccharose and arabinose is not a constant property.

Vibrio anguilarum is isolated from sea and fresh water and from sick fish. Vibrio fischeri from sea water and sea animals, and Vibrio costicola from canned meat and pickles.

The principles of therapy and prophylaxis are the same as those in other toxinfections.

Vibrio vulnificus is a halophilic organism that characteristically produces an overwhelming primary sepsis without an obvious source of infection, or an infection of a preexisting wound followed by a secondary sepsis. Theprimary sepsis seems to follow the ingestion of undercooked or raw seafood, particularly raw oysters. The number of V. vulnificus infections totals fewer than 100 per year in the United States, but the mortality rate is 45 % to 60 % particularly in individuals with liver disease, or thoses with diabetes, kidney disease and and other ailments affecting immune system. As a result, the CDC have strongly recommended: “Don’t eat raw oysters if you suffer from any kind of liver disease.” Secondary sepsis may also occur after the exposure of wounds to salt water or infectes shellfish.

Vibrio fluvialis is another halophilc that has been isolated from the diarrheal stools of many patients in Bangladesh. It has also been found in coastal waters shellfish on the east and west coasts of the United States. This organism has been reported to produce bott enterotoxin-like substances and an extracellular cytotoxin that kills tissue cells.

Vibrio mimicus, an organism similar to certaion-O1 V. choleras strains, also produces a cholera-like disease and reports indicate that it produces an entcrotoxin thatis indistinguishable from choleragen.

Campylobacter. Members of the genus Campyhbacter are gram-negative, curved, spiral rods possessing a single polar flagellum. Four acknowledged species of Campylobacter exist, and several additional species have been termed Campylobacter-like organisms. All seem to be inhabitants of the gastrointestinal tract of wild and domestic animals, including household pets. Transmission to humans occursby a fecal-oral route, originating from farm animals, birds, cats, dogs, and particularly processed poultry. Fifty percent to 70% of all human infections result from handlingor consuming improperly prepared chicken. Because the organisms often are found in unpasteurized milk, many epidemics of campylobacteriosis have been spread via milk. Some epidemics have occurred in school children who were given unpasteurized milk during field trips to dairies. The Food and Drug Administration has, therefore, specifically recommended that childreot be permitted to sample raw milk during such visits.

Campylobacter jejuni ranks along with rotaviruses and ETEC as the major cause of diarrheal disease in the world, particularly in developing countries. Clinical isolates of this organism have been shown to produce a heat-labile enterotoxin that raises intracellular levels of cAMP. Furthermore, the activity of this enterotoxin is partially neutralized by antiserum against E. coli LT and CT, demonstrating that Campylobacter enterotoxin belongs to this same group of adenylate cyclase-activating toxins. The production of this cholera-like toxin does not, however, explain the mechanism by which C. jejuni causes an inflammatory dysentery or bloody diarrhea. Analysis ofstrains producing such infections have revealed the presence of an additional cytotoxin that is biologically distinct from Shiga-like and Clostridium difficile toxins. The role of this toxin as a cause of inflammatory colitis, however, remains unknown. As is true for most intestinal pathogens, C jejuni has been shown to possess an adhesin for intestinal mucosa.

A number of reports have also indicated a close association between certain serotypes of C. jejuni and Guillain-Barre syndrome, but the nature of this relationship is completely unknown. In one study of 46 patients with Guillain-Barre syndrome, C. jejuni was isolated from 30% of patients compared with 1% of controls. Of these, 83% were serotype 19 and 17% were serotype 2.

Campylobacter fetus also causes human diarrheal disease, but this species is more likely to progress to a systemic infection resulting in vascular necrosis.

The incubation period for the diarrheal disease usually is 2 to 4 days. The organisms can be grown readily on an enriched medium under microaerophilic conditions (6% O₂ and 10% CO₂). Gentamicin, erythromycin, and a number of other antibiotics may be used successfully for the treatment of Campylobacter infections.

Surprisingly, H. pylori infection is widespread, particularly in developing countries where it occurs at a younger age than in developed countries. For example, the prevalence of H. pylori infection in Guangdong Province in China was 52.4%, and it has been suggested that earlya cquisition and, hence, long-term infection may be animportant factor predisposing to gastric cancer.

Adhesins, proteases, and cytotoxins all have been reported as virulence factors for H. pylori. One adhesin that has definitely been characterized is the blood group antigen, Lewis^b, (Le^b) which, if present, is found on the surface of gastric epithelial cells in the stomach. Gastric tissue lacking Le^b antigen or antibodies to the Le^b antigen inhibited bacterial binding. Thus, because Le^b is part of the antigen that determines blood group A, individuals with blood group O run a greater risk for developing gastric ulcers. A second adhesin reported to occur on the surface of H. pylori binds specifically to the monosacchande sialic acid, also found on glycoproteins on the surface of gastricepithelial cells.

The production of a cytotoxin that induces vacuolation of eucaryotic cells has been reported to occur inabout 50% of all isolates. Interestingly, one small study suggested that infection with toxin-producing strains was associated with increased antral inflammation.

All wild-type strains of H. pylori do produce the enzyme urease, and a number of reports have indicated that urease may protect the organisms from the acidic environment of the stomach by the release of ammonia from urea. Urease may also function as a cytotoxin, dc- stroying gastric cells that are susceptible to its activity.

Notice that over-the-counter medications containing bismuth salts have been used for years to treat gastritis (Pepto-Bismol, Procter & Gamble Pharm., Norwich, NY) and the fact that H. pylori is sensitive to bismuth may explain its efficacy for the relief of gastric symptoms.

Plesiomonas shigelloides. Plesiomonas shigelloides has been implicated as a cause of diarrhea in the United States as well as in tropical and subtropical countries. The mechanism by which this organism causes diarrhea is unknown, but a report indicated that sterile nitrates of growth medium obtained from 24 different strains of P. shigelloides induced the synthesis of cAMP in Chinese hamster ovary cells. Moreover, this effect was eliminated by either heating the filtrates or by preincubation of them with cholera antitoxin, suggesting that the diarrhea produced by P. shigelloides results from the formation of a cholera-like toxin.

Aeromonas. Aeromonas species are gram-negative, facultatively anaerobic bacteria that are found in soil, fresh and brackish water, and as pathogens of fish, amphibians, and mammals; symptoms range from diarrhea in piglets to fatal septiccmia in fish and dogs and abortion in cattle. Human infections are most commonly seen as a gastroenteritis but Aeromonas organisms have also been recovered from wounds and soft tissue abscesses that have been contaminated with soil or aquatic environments.

In 1988, California became the first state to make infections by Aeromonas a reportable condition and during that year 280 infections were reported, of which 81% were gastroenteritis and 9% were wound infections. Others were isolated from blood, bile, sputum, and urine, occurring mostly in persons with chronic underlying diseases.

Virulence factors that have been reported for Aeromonas include cholera-like and heat stable entcrotoxins andat least two hemolysins, one or both of which may be cytotoxic or enterotoxic. Aeromonas hydrophila and Aeromonas sobria probably are the only clinically important species.

Additional materials for diagnosis

CHOLERA

Cholera is a particularly dangerous infectious disease, caused by Vibrio cholerae and Vibrio El Tor biovars, which runs as gastroenteritis associated with dehydration.

The main method in the laboratory diagnosis of cholera is bacteriological examination. Some 10-20 ml of faeces and vomited matter from patients with suspected cholera are collected with a sterile metallic or wooden spoon, transferred into a sterile wide-mouthed vessel, and tightly stoppered with a-glass or cork plug.

The second portion of faeces and vomit (1-2 ml) is inoculated into 1 per cent peptone water (50 ml) at the patient’s bedside. Both vessels are sealed and immediately sent to the laboratory.

If the patient has no bowel movements at the moment of material collection, cut off soiled samples of the bed linen or underclothes and collect the contents of the rectum with a sterile wire loop inserted 5-8 cm deep. Following removal, put the loop with a faecal sample into a flask with a nutrient medium. At autopsy the material to be tested is obtained in the following manners mark off three sites in the area of the upper, middle and lower portions of the small intestine and a site of the rectum some 10-15 cm long; then from each end of the marked section express the contents of the intestine side-wise, apply two ligatures, and cut an intestine between them. The gallbladder is removed with a part of the liver. The water (1 L) and foodstuffs (no less than 200 g) should also be examined.

In examining convalescents, individuals who have contacted with patients or carriers, it is recommended that a purgative or a cholagogue (25-30 g of magnesium sulphate, etc.) be preliminary given to them to obtain liquid faeces from the upper part of the intestines and the contents of the gallbladder.

The material to be studied is collected, packed, and sent to the laboratory with special measures of precaution. The glassware should not contain any traces of disinfectants, particularly of acids; it is sterilized or boiled for 15 min.

Jars and test tubes should be closed with glass or rubber stoppers. When cork plugs are used, cellulosic film is placed under them. After the material has been collected, the plugs are sealed with paraffin and wrapped with double cellulosic film.

On each vessel stick on a slip of paper with the name and age of the patient, his or her home and office address, diagnosis, the dates of the onset of the disease and hospitalization, as well as the date and exact time of material collection, and also the name of the person who has sent in the analysis.

The material should be brought to the laboratory no later than six hours after its collection. If the delivery within this period is impossible, the samples are inoculated into 1 per cent peptone water with potassium tellurite and onto plates with alkaline agar. If the laboratory is a long way off, jars and test tubes with the specimens to be tested are put, packing them with saw dust, into a metallic container which, in turn, is packed into a wooden box. The latter is wrapped, sealed, signed “Top, fragile”, and is sent with a courier.

Bacteriological examination. Stage I. Using the material collected, prepare smears, dry them in the air, fix with alcohol or Nikiforov’s mixture, stain by the Gram technique, and examine under the microscope. Later on, if laboratory findings confirm the diagnosis of cholera in at least one case, stain the smears with Pfeiffer’s fuchsine only. Cholera vibrios appear as thin curved Gram-negative rods (Fig. 15). Because of great polymorphism the smear may, along with typical cells, contain coccal, rod-shaped, and spiral forms, which diminishes the value of this method.

The first preliminary answer is given after the microscopic examination of the smear. It refers to the presence of vibrios and the nature of their Gram-staining.

At this stage of bacteriological examination, one can also perform the immunoftuorescence test, using specific labelled 0-cholera sera. Moreover, the cholera vibrio may be recovered by the immune Indian ink method. In the latter case smears fixed on a glass slide are treated for 2 min in a humid chamber with Indian ink mixed with immune serum, then washed with water and examined with a microscope. The vibrio is stained black by Indian ink: the walls of the cell are black-brown, the centre is slightly greyish. If the bacteria are few, they are preliminarily cultivated for 3-5 hrs in peptone water.

Simultaneously with bacterioscopy, the material tested is inoculated onto liquid and solid nutrient media. Enrichment liquid media that are usually recommended for use include alkaline 1 per cent peptone water, 1 per cent peptone water with potassium tellurite in a ratio of 1 to 100 000, and alkaline taurocholate-tellurite-peptone medium (Monsur’s liquid medium), etc.

Solid nutrient media usually employed are alkaline meat-peptone agar and one of the selective nutrient media: Aronson’s medium, Monsur’s alkaline taurocholate-lellurite-gelatine-agar medium, TCBS, etc.

To isolate the vibrio from carriers or patients with subclinical forms of cholera, use media which improve the growth of vibrios and suppress the attendant flora (predominantly E. coli). All inoculated cultures are placed in an incubator at 37 °C.

Aronson’s medium consists of 2-3 per cent of meat-peptone agar to which sucrose and destained fuchsine are added.

Monsur’s alkaline taurocholate-tellurite-gelatine-agar medium contains 10 g of trypticase, l0 g of sodium chloride, 50 g of sodium taurocholate, 30g of sodium carbonate, 1 g of gelatin, 15 g of agar-agar, and 1 L of distilled water.

TCBS (thiosulphate-citrate-bromthymol sucrose) is manufactured in the form ready for use; 69 g of the dry medium is taken per 1 L of distilled water.

Stage II. Some 5-6 hours after inoculation examine the film on the peptone water. To do it, tilt the test tube or the vial so that a delicate bluish film is attached to the wall. Prepare smears from the film or the surface of the medium, stain them by the Gram method, evaluate motility, and conduct presumptive slide agglutination test with 0-cholera (0-1) serum diluted 1:100 or the reaction of cholera vibrio immobilization with 0-cholera serum. The results of the latter are estimated by phase-contrast microscopy. Inhibition of vibrios motility and the formation of agglutinate occur within 1-2 min.

On the basis of the results obtained give a second preliminary result referring to the motility of the vibrio and its relation to the agglutinating serum.

Subculture the material from the film onto plates with alkaline agar or selective medium and simultaneously onto the second peptone water and look for changes in 5-6 hrs.

Stage III. Some 10-16 hrs after inoculation, examine the growth in the second enrichment medium (peptone water) and on the plates with the culture of the native material. The film formed on the peptone water is examined as described above.

On an alkaline agar the cholera vibrio grows with the formation of round, smooth, flat, bluish, homogeneous colonies which are 1–2 mm in diameter, transparent in the transmitted light and have smooth edges. They are oily in consistence, are readily removed and emulsified. Examination of the material from convalescents, bacteria carriers, and individuals treated with antibiotics may reveal atypical colonies.

The selected colonies are introduced into test tubes with Oikenitsky’s medium or onto an agar slant for enrichment of pure culture and placed in an incubator.

Preliminary identification of cholera vibrios grown on plates with solid media is based on the study of cultural and morphological characteristics and on a presumptive slide agglutination test with 0-cholera serum diluted 1:100 and with Ogawa’s and Inaba’s sera in a 1 to 60 dilution, which is carried out to determine the serovar.

If the examination demonstrates signs typical of the cholera vibrio, a third preliminary answer about the positive result of the investigation is issued. Some material from the typical colonies may be transferred to a broth; then, using a 3-4-hour old culture, perform a standard agglutination test, check fermentation of carbohydrates, and determine whether the isolated culture belongs to Group I according to Heiberg and whether it is liable to phagolysis by cholera phages C and El Tor 2. If the results are positive, an answer concerning the isolation of the causative agent is given within 18-24 hrs from the beginning of the study.

Stage IV. After the results of the standard agglutination test and the reaction of phagolysis and fermentation of carbohydrates by 3-4-hour broth culture have been analysed, a preliminary conclusion about the isolation of the cholera vibrio is made. Plates with the inoculated culture on the second peptone water are examined, using the scheme which is employed in examining the plates with the culture of the native material. On Oikenitsky’s medium the vibrio breaks down sucrose without gas formation and does not ferment lactose (reddening of the medium in the column without gas formation).

To distinguish vibrios from homogeneous species of microorganisms (Aeromonas, Pseudomonas, Plesiomonas), a number of tests may be employed: the oxidase test, glucose oxidation-fermentation reaction, the “strand” test (Table ).

Table

Differential-Diagnostic Signs of Vibrios and Related Types of Bacteria

Microorganisms

Osidase test

Glucose reduction-fermentation

“Strand” test

reduction

fermentation

Vibrio

++++

+ (gas is absent)

Aeromonas

++++

+ (gas ±)

Pseudomonas

–

Plesiomonas

–

The oxidase test consists of placing a solution of paraaminodimethyl–aniline and alpha-naphthol onto the culture in a Petri dish or onto a meat-peptone agar slant.

To carry out the oxidation-fermentation test, medium with the following composition (per 100 ml) is prepared: 2.0 g of peptone; 5.0 g of sodium chloride; 0.3 g of potassium hydrophosphate; 3.0 g of agar-agar; bromthymol blue (1 per- cent aqueous solution).

Dispense the medium in 3-4-ml portions into 13 X 100 mm test tubes and sterilize for 15 min at 120 °C. After that, add to the tubes 10 per cent glucose solution sterilized by nitration to adjust to the final concentration of 1 per cent. Inoculate the test culture into two tubes with the above mentioned medium- Into one of the tubes pour a layer (1.5-2 cm) of sterile petrolatum oil. Incubate the test tubes for four days and note acid and gas formation. Darkening of the medium in the open test tube indicates oxidation and in the tube with the oil, fermentation. Gas formation is sometimes observed.

The “strand” test. Onto a glass slide, place a drop of 0.5 per cent solution of sodium desoxycholate in buffer isotonic saline. Into this drop, introduce a loop-ful of the tested culture of vibrios grown on a solid nutrient medium and mix. If the result is positive, the mixture becomes transparent, acquires mucilaginous consistency, and trails the loop in the form of a strand in the first minutes after its preparation.

To differentiate between the classical cholera vibrio and the El Tor vibrio, utilize tests determining the sensitivity of cholera vibrios toward phage’s and polymixin and the ability of vibrios to agglutinate chick erythrocytes.

Sensitivity to diagnostic phages is determined by streaking onto a plate with a culture of whole cholera phages C and EI Tor 2, with ten-fold dilutions of the above. Phage C is active only toward the classic cholera vibrio, while the El Tor 2 phage is active toward El Tor biovar. The presence of lysis in the form of one “sterile” spot or a group of small spots in the place of phage introduction is assessed as a positive result.

Sensitivity to polymixin is determined by inoculating the isolated culture onto Petri dishes with nutrient agar containing 50 U of polymixin M or B in 1 ml of nutrient medium. El Tor vibrios are insensitive to antibiotics and show good growth on the dishes, unlike the classic cholera vibrios.

Haemagglutination of chicken erythrocytes is performed on a glass slide. In a drop of isotonic sodium chloride solution, comminute a loopful of 18-hour culture of the vibrio and add a drop of 2.5 per cent suspension of chicken erythrocytes. The cholera El Tor vibrio agglutinates the red blood cells within 1-3 min, whereas the classical biovar fails to induce any clumping.

Haemolysis of sheep erythrocytes (Greig’s test) occurs after their 2-hour incubation with broth culture of cholera El Tor vibrios at 37 °C. Yet, this sign is not stable and some strains of the El Tor biovar, similar to the classical cholera vibrio, display no haemolytic effect.

The Voges-Proskauer test is based on the ability of El Tor vibrios to form acetylmethylcarbinol, which is recognized by the fact that Clark’s glucose-phosphate broth becomes pink or ruby-red, following 1-3 day incubation of the inoculated cultures with addition of alpha-naphthol.

The hexamine test is performed with 24-hour broth culture of the vibrio a loopful of which is streaked onto 1 ml of a glucose-hexamine medium. Following incubation at 37 °C for 6-24 hrs, the El Tor vibrio alters the colour of the medium from green to yellow. The classical cholera vibrio induces no changes in the medium colour over this time.

Production of enterotoxin by the cholera vibrio is determined by means of a specific reaction of passive immune haemolysis. To carry out this reaction, re-suspend in 0.08 M phosphate buffer the erythrocytes from defibrinated sheep blood (after their triple washing). Prepare 10 per cent suspension of red blood cells in 0.02 M solution of the buffer. With a micropipette introduce 0.025 ml portions of 0.02 M phosphate buffer into agglutinating plates, then add twofold dilutions of the antigen and 1 per cent suspension of erythrocytes. Cover the plates and place them into a 37 °C incubator for 30 min, add to each well (1.025 ml of antitoxic serum diluted 1:50 and 0.02 per cent bovine serum albumin, reincubate the culture for 30 min, then add 0.025 ml of complement and replace the culture into the incubator for 90 min. .Haemolysis is evaluated after 30 min of keeping the plates at room temperature. Simultaneously, one monitors non-immune haemolysis and the ingredients of the serum, antigen, and complement.

Isolation of non-agglutinating vibrios brings about the necessity of studying their biochemical properties, namely: liquefaction of gelatine, splitting of 5tarch (Cadamot’s test), formation of indol from triptophane, reduction of nitrates into nitrites, as well as oxidase and decarboxylase activity. It is also necessary to classify the culture with one of the biochemical groups according to Heiberg.

Demonstration of cholera vibrios in water is of great importance for identifying the factors of infection transmission and conducting anti-epidemic measures. Using a saturated solution of sodium hydrocar-bonate, alkalize the water (900 ml) delivered to the laboratory to pH of 7.8-8.0, add 100 ml of basic peptone, pH 8.0 (peptone, 100 g, sodium chloride, 50 g, potassium nitrate, 1 g, sodium hydrocarbon-ate, 20 g, distilled water, 1000 ml), and dispense it into flasks or vials in 100-200-ml portions. Incubate the inoculated cultures at 37 °C for 5-8 hrs and then inspect them in the manner employed for studying other inoculated cultures in peptone water (vomited matter and faeces). The results are more reliable when the water tested is filtered through membrane filters. Large amounts of water (1.5-2.5 1) are examined and the deposit from the filters is transferred to peptone water (pH 8.0) and alkaline agar.

Rapid detection of cholera vibrios in drinking water. If water contamination with cholera vibrios is heavy (at least 100 vibrios per 1 ml), the agglutination reaction is utilized for their recovery. To the water to be assayed add weakly alkaline concentrated solution of peptone in a quantity sufficient to produce 1 per cent solution. With this mixture dilute the 0-cholera agglutinating serum from 1:100 to its titre. Use a mixture free of the serum as a control. Place the test tubes into an incubator and read the results of the test in 6 hrs. The reaction is considered positive it flocculation is observed upon serum dilution to half the litre or the litre.

Rapid recovery of the cholera vibrio in water may also be based on increase in the phage titre.

Rapid method of wide-scale screening for carriers. During an outbreak of cholera wide-scale screening for carriers of the cholera vibrio is performed. When a large number of analyses is to be made in the laboratory, faeces from ten subjects are examined simultaneously. Faeces are collected with wire loops and placed into one flask containing 200 ml of peptone water and 0-cholera agglutinating serum which is diluted to half the titre. The flask is placed into a 37 “C incubator. In 3-4 hrs the multiplied cholera vibrios begin to agglutinate and fall to the bottom in the form of flakes. If this is the case, faecal material is taken from each of the ten individuals, and the examination is repeated with each sample.

Serological diagnosis of cholera is supportive and relies on detecting agglutinins and vibriocidal antibodies in the patient’s serum. It is recommended that paired sera obtained from the patients at a 6-8 day interval be used for these reactions. Titres of agglutinins and vibriocidal antibodies usually tend to increase simultaneously. The most sensitive test is demonstration of vibriocidal antibodies. The presence of agglutinating antibodies in the titre of 1:80-1:320 and vibriocidal ones in the titre of 1:1000 is considered diagnostically positive.

Campylobacter

Campylobacter (meaning ‘twisted bacteria’) is a genus of bacteria that are Gram-negative, spiral, and microaerophilic. Motile, with either unipolar or bipolar flagella, the organisms have a characteristic spiral/corkscrew appearance (see photo) and are oxidase-positive.[1] Campylobacter jejuni is now recognized as one of the main causes of bacterial foodborne disease in many developed countries.[2] At least a dozen species of Campylobacter have been implicated in human disease, with C. jejuni and C. coli the most common.[1] C. fetus is a cause of spontaneous abortions in cattle and sheep, as well as an opportunistic pathogen in humans.[3]

The genomes of several Campylobacter species have been sequenced, providing insights into their mechanisms of pathogenesis.[4] The first Campylobacter genome to be sequenced was C. jejuni, in 2000.[5]

Campylobacter species contain two flagellin genes in tandem for motility, flaA and flaB. These genes undergo intergenic recombination, further contributing to their virulence.[6] Nonmotile mutants do not colonize.[c

Campylobacteriosis is an infection by Campylobacter.^[8] The common routes of transmission are fecal-oral, ingestion of contaminated food or water, and the eating of raw meat. It produces an inflammatory, sometimes bloody, diarrhea, periodontitis ^[9] or dysentery syndrome, mostly including cramps, fever and pain. The infection is usually self-limiting and in most cases, symptomatic treatment by liquid and electrolyte replacement is enough in human infections. The use of antibiotics, on the other hand, is controversial. Symptoms typically last for five to seven days.^[^citatioeeded^]

Cause

The sites of tissue injury include the jejunum, the ileum, and the colon. Most strains of C jejuni produce a toxin (cytolethal distending toxin) that hinders the cells from dividing and activating the immune system. This helps the bacteria to evade the immune system and survive for a limited time in the cells. A cholera-like enterotoxin was once thought to be also made, but this appears not to be the case. The organism produces diffuse, bloody, edematous, and exudative enteritis. Although rarely has the infection been considered a cause of hemolytic uremic syndrome and thrombotic thrombocytopenic purpura, no unequivocal case reports exist. In some cases, a Campylobacter infection can be the underlying cause of Guillain–Barré syndrome. Gastrointestinal perforation is a rare complication of ileal infection.^[1

campylobacter Questions and Answers

“Campylobacter” bacteria are the second most frequently reported cause of foodborne illness. A comprehensive farm-to-table approach to food safety is necessary in order to reduce campylobacteriosis. Farmers, industry, food inspectors, retailers, food service workers, and consumers are each critical links in the food safety chain. This document answers common questions about the bacteria “Campylobacter,” describes how the Food Safety and Inspection Service (FSIS) of the U.S. Department of Agriculture (USDA) is addressing the problems of “Campylobacter” contamination on meat and poultry products, and offers guidelines for safe food handling to prevent bacteria, such as “Campylobacter,” from causing illness.

Q. What is Campylobacter?
A. Campylobacter [pronounced “kamp-e-lo-back-ter”] is a gram negative, microaerophilic bacterium and is one of the most common bacterial causes of diarrheal illness in the United States. Campylobacter jejuni, the strain associated with most reported human infections, may be present in the body without causing noticeable illness.

Campylobacter organisms can be found everywhere and are commonly found in the intestinal tracts of cats, dogs, poultry, cattle, swine, rodents, monkeys, wild birds, and some humans. The bacteria pass through the body in the feces and cycle through the environment. They are also found in untreated water.

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Q. What harm can Campylobacter bacteria cause?
A. Infection caused by Campylobacter bacteria is called campylobacteriosis and is usually caused by consuming unpasteurized milk, raw or undercooked meat or poultry, or other contaminated foods and water, and contact with feces from infected animals. While the bacteria can exist in the intestinal tracts of people and animals without causing any symptoms or illness, studies show that consuming as little as 500 Campylobacter cells can cause the illness.

Symptoms of Campylobacter infection, which usually occur within 2 to 10 days after the bacteria are ingested, include fever, abdominal cramps, and diarrhea (often bloody). In some cases, physicians prescribe antibiotics when diarrhea is severe. The illness can last about a week.

Complications can include meningitis, urinary tract infections, and possibly reactive arthritis (rare and almost always short-term), and rarely, Guillain-Barre syndrome, an unusual type of paralysis. While most people who contract campylobacteriosis recover completely within 2 to 5 days, some Campylobacter infections can be fatal, resulting in an estimated 124 deaths each year.

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Q. Are more people becoming ill from campylobacteriosis?
A. The Foodborne Diseases Active Surveillance Network (FoodNet) found a decline, in the rates of infection in 2009 for Campylobacter (30% decrease), in comparison with the previous three years of surveillance (1996 to 1998). Still, according to the Centers for Disease Control and Prevention (CDC), campylobacteriosis causes an incidence of about 13 cases per 100,000 population diagnosed in the United States annually.

FoodNet is a collaborative project among CDC, the 10 Emerging Infections Program sites (EPIs), USDA, and the U.S. Food and Drug Administration (FDA). One of the objectives of FoodNet is to measure effectiveness of a variety of preventive measures in reducing the incidence of foodborne illness attributable to the consumption of meat, poultry, and other foods.

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Q. Who is most susceptible?
A. Anyone may become ill from Campylobacter. However, infants and young children, pregnant women and their unborn babies, and older adults, are at a higher risk for foodborne illness, as are people with weakened immune systems (such as those with HIV/AIDS, cancer, diabetes, kidney disease, and transplant patients).

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Q. How can Campylobacter be controlled?
A. Campylobacter can be controlled at a number of different points in the food production and marketing chain.

On the farm:

Good sanitary practices on farms, as recommended by USDA, minimize the opportunity for the bacteria to spread among animals and birds.
Pasteurization of milk and treatment of municipal water supplies eliminate another route of transmission for Campylobacter and other bacteria.

In the plant:

Raw foods are not sterile, and there are no requirements that they be sterile. Food processing companies are accountable for following good, up-to-date manufacturing practices that minimize the opportunity for the spread of Campylobacter and other bacteria.

At retail:

A food recall is a voluntary action by a manufacturer or distributor to protect the public from products that may cause health problems or possible death. FSIS conducts a sufficient number of effectiveness checks to verify the recalling firm has contacted the distributor or retailer.

Individuals:

Reporting the problem is another way to control these bacteria and prevent others from becoming exposed to the source of contamination. Any individual that experiences symptoms of campylobacteriosis should contact a physician. Physicians who diagnose campylobacteriosis and clinical laboratories that identify this organism should report their findings to the local health department.

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Q. What is FSIS doing to prevent Campylobacter infections?
A. In its commitment to ensure that the public has a safe, wholesome food supply, FSIS is constantly working to improve the level of safety and reduce contaminants in the meat and poultry supply.

In 1998, FSIS began enforcing a combination of Hazard Analysis and Critical Control Points (HACCP) based process control, microbial testing, pathogen reduction performance standards, and sanitation standard operating procedures which significantly reduce contamination of meat and poultry with harmful bacteria and reduce the risk of foodborne illness. Establishments can choose to include Campylobacter in their HACCP analysis. If Campylobacter is identified by the establishment as being reasonably likely to occur or if it becomes evident that it is an emerging problem in their process, FSIS would expect the establishment to have controls in place designed to address this microbial food safety hazard.

HACCP clarifies the responsibilities of industry and FSIS in the production of safe meat and poultry products. The role of FSIS is to set appropriate food safety standards and maintain vigorous inspection oversight to ensure that those standards are met.

USDA is supporting research to learn more about Campylobacter in food and how to control it.

Finally, FSIS maintains extensive safe food handling education programs to help individuals prevent and reduce the risks of foodborne illness.

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Q. What is the best way to prevent Campylobacter infections?
A. Meat and poultry can contain Campylobacter. However, the bacteria can be found in almost all raw poultry because it lives in the intestinal track of healthy birds. Improving safe food handling practices in kitchens will reduce the number of Campylobacter illnesses. Campylobacter bacteria are extremely fragile and are easily destroyed by cooking to a safe minimum internal temperature. They are also destroyed through typical water treatment systems. Freezing cannot be relied on to destroy the bacteria. Home freezers are generally not cold enough to destroy bacteria.

To destroy Campylobacter and minimize the risk of foodborne illnesses:

CLEAN: Wash Hands and Surfaces Often

Wash your hands with warm soapy water for 20 seconds before and after handling food and after using the bathroom, changing diapers, and handling pets.
Wash utensils, cutting boards, dishes, and countertops with hot soapy water after preparing each food item and before you go on to the next item.
Consider using paper towels to clean kitchen surfaces. If you use cloth towels, wash them often in the hot cycle of your washing machine.

SEPARATE: Don’t Cross-contaminate

Separate raw meat, poultry, and seafood from other foods in your grocery shopping cart and in your refrigerator.
If possible, use one cutting board for fresh produce and a separate one for raw meat, poultry, and seafood.
Always wash cutting boards, dishes, countertops, and utensils with hot soapy water after they come in contact with raw meat, poultry, and seafood.
Never place cooked food on a plate which previously held raw meat, poultry, or seafood.

COOK: Cook to Safe Temperatures
Use a clean food thermometer when measuring the internal temperature of meat, poultry, casseroles, and other foods to make sure they have reached a safe minimum internal temperature:

Cook all raw beef, pork, lamb and veal steaks, chops, and roasts to a minimum internal temperature of 145 °F (62.8 °C) as measured with a food thermometer before removing meat from the heat source. For safety and quality, allow meat to rest for at least three minutes before carving or consuming. For reasons of personal preference, consumers may choose to cook meat to higher temperatures.
Cook all raw ground beef, pork, lamb, and veal to an internal temperature of 160 °F (71.1 °C) as measured with a food thermometer.
Cook all poultry to a safe minimum internal temperature of 165 °F (73.9 °C) as measured with a food thermometer.
For optimum safety, cook stuffing separately to 165 °F (73.9 °C).
Egg dishes, casseroles to 160 °F (71.1 °C).
Fish should reach 145 °F (62.8 °C) as measured with a food thermometer.
Bring sauces, soups, and gravy to a boil when reheating.
Reheat leftovers thoroughly to at least 165 °F (73.9 °C).

In addition, do not eat or drink foods containing raw, unpasteurized milk.

CHILL: Refrigerate Promptly

Keep food safe at home, refrigerate promptly and properly. Refrigerate or freeze perishables, prepared foods, and leftovers within 2 hours — 1 hour if the temperature is above 90 °F (32.2 °C).
Freezers should register 0 °F (-17.8 °C) or below and refrigerators 40 °F (4.4 °C) or below.
Thaw food in the refrigerator, in cold water, or in the microwave. Foods should not be thawed at room temperature. Foods thawed in the microwave or in cold water must be cooked to a safe minimum internal temperature before refrigerating.
Marinate foods in the refrigerator.
Divide large amounts of leftovers into shallow containers for quick cooling in the refrigerator.
Don’t pack the refrigerator. Cool air must circulate to keep food safe.

For more information about Campylobacter, see the Centers for Disease Control and Prevention (CDC) Web site at: http://www.cdc.gov/nczved/divisions/dfbmd/
diseases/campylobacter/

helicobacter

Helicobacter is a genus of Gram-negative bacteria possessing a characteristic helix shape. They were initially considered to be members of the Campylobacter genus, but since 1989 they have been grouped in their own genus. The Helicobacter genus belongs to class Epsilonproteobacteria, order Campylobacterales, family Helicobacteraceae and already involves >35 species.^[1]^[2]^[3]^[4]

Some species have been found living in the lining of the upper gastrointestinal tract, as well as the liver of mammals and some birds.^[5] The most widely known species of the genus is H. pylori which infects up to 50% of the human population.^[4] Some strains of this bacterium are pathogenic to humans as it is strongly associated with peptic ulcers, chronic gastritis, duodenitis, and stomach cancer. It also serves as the type species of the genus.

Helicobacter spp. are able to thrive in the very acidic mammalian stomach by producing large quantities of the enzyme urease, which locally raises the pH from ~2 to a more biocompatible range of 6 to 7.^[6] Bacteria belonging to this genus are usually susceptible to antibiotics such as penicillin, are microaerophilic (optimal oxygen concentration between 5 – 14%) capnophiles, and are fast-moving with their flagella.^[7]^[8]

Comparative genomic analysis has led to the identification of 11 proteins which are uniquely found in members of the family Helicobacteraceae. Of these proteins, 7 are found in all species of the family, while the remaining 4 are not found in any Helicobacter strains and are unique to Wollinella.^[9] Additionally, a rare genetic event has led to the fusion of the RpoB and RpoC genes in this family, which is characteristic of this family.^[9]^[10]

^V ecently, new gastric (Helicobacter suis and Helicobacter baculiformis) and enterohepatic (Helicobacter equorum) species have been reported. Helicobacter pylori is of primary importance for medicine; however, non-pylori Helicobacter species (NPHS), which naturally inhabit mammals (except humans) and birds, have been detected in human clinical specimens. NPHS encompass two (gastric and enterohepatic) groups, showing different organ specificity. Importantly, some species such as Helicobacter hepaticus, Helicobacter mustelae and, probably, Helicobacter bilis exhibit carcinogenic potential in animals. NPHS harbour many virulence genes and may cause diseases not only in animals but also in humans. Gastric NPHS such as H. suis (most often), Helicobacter felis, Helicobacter bizzozeronii and Helicobacter salomonis have been associated with chronic gastritis and peptic ulcers in humans and, importantly, with higher risk for MALT lymphoma compared to H. pylori. Enterohepatic species e.g., H. hepaticus, H. bilis and Helicobacter ganmani have been detected by PCR in but still not isolated from specimens of patients with hepatobiliary diseases. Moreover, NPHS may be associated with Crohn’s disease, inflammatory bowel disease and ulcerative colitis. The significance of avian helicobacters (Helicobacter pullorum, Helicobacter anseris and Helicobacter brantae) also has been evaluated extensively. NPHS such as Helicobacter cinaedi and Helicobacter canis can cause severe infections, mostly in immunocompromised patients with animal exposure. Briefly, the role of NPHS in veterinary and human medicine is increasingly recognised. However, despite the growing interest in the possible association between NPHS and the chronic hepatobiliary or intestinal diseases in humans, more studies are still required to prove the suggested association. Several other topics such as isolation of still uncultured species, antibiotic resistance and treatment regimens for NPHS i VIBRIO. LABORATORY DIAGNOSIS of CHOLERA. CAmpYlobacter, helicobacter and aeromonas infections

Vibrio cholerae biovar Proteus (N. Gamaleya, 1888) and Vibrio cholerae biovar albensis were discovered” later. V. cholerae was described by F. Pacini in 1854.

Morphology. Cholera vibrios are shaped like a comma or a curved rod measuring 1-5 mcm in length and 0.3 mcm in breadth (Fig.).

Figure. Vibrio cholerae: 1-pure culture; 2- flagellate vibrios

They are very actively motile, monotrichous, nonsporeforming, noncapsulated, and Gram-negative.

The organism is also subjected to cultural changes. It dissociates from the S-form to the R-form, this process being accompanied by profound changes in antigenic structure.

Cholera is undoubtedly the most dramatic of the water-borne diseases. As far as is known, cholera was confined to India for the almost 2000 years between its first description by Hindu physicians in 400 b c and its spread to Arabia, Persia, Turkey, and Southern Russia in the early1800s. There were six major pandemics of cholera during the 1800s covering the entire world, killing millions wherever it struck. During one such outbreak in London during 1849, the famous physician, John Snow, traced the spread of the disease to a Broad Street pump from which area residents obtained their water. The spread of cholera in this area was stopped when Snow recommended that the handle of the pump be removed. This is particularly remarkable when one remembers that the germ-theory of disease had not yet been formulated.

Laboratory diagnosis. A strict regimen is established in the laboratory. Examinations are carried out in accordance with the general rules observed for particularly hazardous diseases.

1. Stool smears stained by a water solution of fuchsin are examined microscopically. In the smears, the cholera vibrios occur in groups similar to shoals of fish (Fig.).

Figure. Vibrio cholerae (stool smear)

The vibrio culture grown on solid media is examined for motility and agglutinable properties. Then it is subcultured on an agar slant to obtain the pure culture.

Table

Differentiation of Biovars of Cholera vibrio

Vibrio

Fermentation within 24 hrs

Seep erythrocyte hemolysis

Lysis by specific O-1 subgroup phages

Agglutination by O-1 cholera serum

Sensitivity to polymixin B

sacharose

mannose

arabinose

Vibrio cholerae biovar cholerae

–

Vibrio cholerae biovar El Tor

–

Vibrio cholerae biovar Proteus

–

Vibrio cholerae biovar albensis

–

Note “A” – carbohydrate fermentation with acid production; “+””– positive result; “–” – negative result; “+” – negative or positive result is not always observed.

An allergy intracutaneous test with Tsuverkalov’s dysenterine is of supplementary significance. It becomes positive in dysentery patients beginning with the fourth day of the disease. The result is read in 24 hrs by the size of the formed papula. The test is considered markedly positive in the presence of oedema and skin hyperaemia 35 mm or more in diameter, moderately positive if this diameter is 20-34 mm, doubtful if there is no papula and the diameter of skin hyperaemia measures 10-15 mm, and negative if the hyperaemic area is less than 10 mm.

Another technique that can be employed is determination of the indicator of neutrophil damage in the presence of dysenterine.

Examination of water, milk, and washings off various objects for Shigellae is conducted utilizing the above mentioned techniques. Of especial importance for examination of these objects is the test aimed at determining the increase in the phage litre, which is also employed for demonstration of Shigella bacteria in the patient’s faeces.

To carry out this test, the indicator phages and reference strains of Flexner’s and Sonne’s Shigella bacteria are used. A rise in the phage titre by 3-5 orders (4-) is considered as weak positive reaction, by 5-7 orders (++) and 7-10 orders (+++), positive, and by over 10 orders (++++), markedly positive.

The immunofluorescence test for Shigella recovery is employed in examining objects containing minor amounts of the causative agents and for rapid laboratory diagnosis of dysentery.